重组小鼠Eph receptor A1/EphA1蛋白(ab55900)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
-
产品名称
重组小鼠Eph receptor A1/EphA1蛋白
参阅全部 Eph receptor A1/EphA1 蛋白酶 -
生物活性
Specific activity : 32 nmol/min/mg.
-
纯度
> 85 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Mouse -
预测分子量
71 kDa -
氨基酸
569 to 977 -
标签
GST tag N-Terminus -
额外的序列信息
Recombinant fragment, corresponding to amino acids 569-end of Mouse Eph receptor A1.
-
相关产品
-
Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab55900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
SDS-PAGE
-
形式
Liquid -
补充说明
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
Previously labelled as Eph receptor A1.
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- EPH
- EPH receptor A1
- EPH tyrosine kinase
see all -
功能
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis. -
组织特异性
Overexpressed in several carcinomas. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
翻译后修饰
Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
Ubiquitinated. -
细胞定位
Cell membrane. - Information by UniProt
图片
-
Functional Studies - Recombinant mouse Eph receptor A1/EphA1 protein (ab55900)The specific activity of Eph receptor A1/EphA1 (ab55900) was determined to be 36 nmol/min/mg as per activity assay protocol
-
SDS-PAGE - Recombinant mouse Eph receptor A1/EphA1 protein (ab55900)SDS PAGE analysis of ab55900 -
Functional Studies - Recombinant mouse Eph receptor A1/EphA1 protein (ab55900)Kinase assay using ab55900. -
SDS-PAGE - Recombinant mouse Eph receptor A1/EphA1 protein (ab55900)ab55900 on SDS-PAGE.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab55900 尚未被引用在任何文献中。