重组小鼠DRAK2蛋白(Active) (ab271491)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, WB
描述
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产品名称
重组小鼠DRAK2蛋白(Active)
参阅全部 DRAK2 蛋白酶 -
生物活性
Specific Activity: 5 pmole/min/μg
Assay Conditions: Reaction was performed in 50 mM Tris pH 7.4, 5 mM MgCl2, 1 mM DTT, 20 μM ATP, 0.2 mg/ml MRCL3 Peptide. Reaction was initiated with the addition of Mouse DRAK2 and was incubated at 30°C for 45 min. The luminescence was read with the Kinase Glo Max reagent.
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纯度
>= 75 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
SRRRFDCRSV SGLLTTTPQT PIKTENFNNF YTLTPKELGR GKFAVVRQCI SKSTGQEYAA KSLKKRRRGQ DCRAEILHEI AVLELARSCP HVINLHEVYE NATEIILVLE YAAGGEIFNL CLPELAEMVS ENDVIRLIKQ ILEGVHYLHQ NNIVHLDLKP QNILLSSIYP LGDIKIVDFG MSRKIGNASE LREIMGTPEY LAPEILNYDP ITTATDMWNI GIIAYMLLTH TSPFVGEDNQ ETYLNISQVN VDYSEEMFSS VSQLATDFIQ SLLVKNPEKR PTAESCLSHS WLQQWDFGSL FHPEETSGSS QIQDLTLRSS EEKTSKSSCN GSCGAREDKE NIPEDGSLVS KRFRFDDSLP SPHELVPDLF C -
预测分子量
68 kDa including tags -
氨基酸
2 to 372 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab271491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 1.19% HEPES, 0.001% Polyoxyethylene lauryl ether, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.13% Magnesium chloride, 1.13% Potassium chloride, 5% Glycerol (glycerin, glycerine), 0.003% EDTA, 0.03% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- DAP kinase related apoptosis inducing protein
- DAP kinase related apoptosis inducing protein kinase 2
- DAP kinase-related apoptosis-inducing protein kinase 2
see all -
功能
Acts as a positive regulator of apoptosis. -
组织特异性
Highly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Nucleus. - Information by UniProt
图片
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Functional Studies - Recombinant mouse DRAK2 protein (Tagged) (ab271491)
Specific activity of ab271491 was 5 pmole/min/μg
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SDS-PAGE - Recombinant mouse DRAK2 protein (Tagged) (ab271491)SDS-PAGE analysis of ab271491.
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Western blot - Recombinant mouse DRAK2 protein (Tagged) (ab271491)All lanes :
Lane 1 : ab271491
Lane 2 : MW markers
Predicted band size: 42 kDaWeastern blot analysis of ab271491.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271491 尚未被引用在任何文献中。