重组小鼠DLK-1蛋白(Fc fragment) (ab108574)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组小鼠DLK-1蛋白(Fc fragment)
参阅全部 DLK-1 蛋白酶 -
生物活性
Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells. -
纯度
> 90 % SDS-PAGE.
ab108574 is 0.2µm filtered -
内毒素水平
< 0.100 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
预测分子量
75 kDa -
氨基酸
1 to 305 -
标签
Fc tag C-Terminus -
额外的序列信息
Fused with the Fc region of Human IgG1 at the C-terminus.
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技术指标
Our Abpromise guarantee covers the use of ab108574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
补充说明
Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: PBS
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复溶Reconstitute with 100µl sterile water.
常规信息
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别名
- Adipocyte differentiation inhibitor protein
- Brevideltin
- Delta like 1 homolog
see all -
功能
May have a role in neuroendocrine differentiation. -
组织特异性
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
序列相似性
Contains 6 EGF-like domains. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
图片
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Functional Studies - Recombinant Mouse DLK-1 protein (Fc fragment) (ab108574)
Induction of SOX-9 with the treatment of recombinant mouse DLK-1-Fc (ab108574).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5µg/ml of ab108574 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-rabbit Sox-9 and anti-rabbit GAPDH.1=0 min; 2=10 min; 3=30 min; 4=1 h; 5=2 h; 6=3 h; 7=4 h; 8=24 h.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab108574 尚未被引用在任何文献中。
