重组小鼠Cathepsin D蛋白(Active) (ab224786)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组小鼠Cathepsin D蛋白(Active)
参阅全部 Cathepsin D 蛋白酶 -
生物活性
Specific activity is > 1,000 pmol/min/µg in which one unit will convert 1.0 pmole of MCA-PLGL-Dpa-AR-NH2 to MCA-Pro-Leu-OH per minute at pH 3.5 at 25°C.
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纯度
> 90 % SDS-PAGE.
Affinity purified -
内毒素水平
< 1.000 Eu/µg -
表达系统
Baculovirus infected insect cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
IIRIPLRKFTSIRRTMTEVGGSVEDLILKGPITKYSMQSSPKTTEPVSEL LKNYLDAQYYGDIGIGTPPQCFTVVFDTGSSNLWVPSIHCKILDIACWVH HKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCKSDQSKARGI KVEKQIFGEATKQPGIVFVAAKFDGILGMGYPHISVNNVLPVFDNLMQQK LVDKNIFSFYLNRDPEGQPGGELMLGGTDSKYYHGELSYLNVTRKAYWQV HMDQLEVGNELTLCKGGCEAIVDTGTSLLVGPVEEVKELQKAIGAVPLIQ GEYMIPCEKVSSLPTVYLKLGGKNYELHPDKYILKVSQGGKTICLSGFMG MDIPPPSGPLWILGDVFIGSYYTVFDRDNNRVGFANAVVLLEHHHHHH -
预测分子量
44 kDa including tags -
氨基酸
21 to 410 -
标签
His tag C-Terminus -
额外的序列信息
This product is the mature full length protein from aa 21 to 410 including the propeptide. The signal peptide is not included (NP_034113).
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab224786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 10% Glycerol (glycerin, glycerine), PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CatD
- CATD_HUMAN
- Cathepsin D
see all -
功能
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
组织特异性
Expressed in the aorta extrcellular space (at protein level). -
疾病相关
Ceroid lipofuscinosis, neuronal, 10 -
序列相似性
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
图片
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SDS-PAGE - Recombinant mouse Cathepsin D protein (Active) (ab224786)
15% SDS-PAGE analysis of 3 µg ab224786.
MW: 40-57 kDa (SDS-PAGE under reducing conditions).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224786 尚未被引用在任何文献中。