Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies
参阅全部 Caspase-1 蛋白酶
Specific Activity: >2000 units/mg.
Unit definition: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
纯度> 80 % SDS-PAGE.
蛋白长度Full length protein
序列NK EDGTFPGLTG TLKFCPLEKA QKLWKENPSE IYPIMNTTTR TRLALIICNT EFQHLSPRVG AQVDLREMKL LLEDLGYTVK VKENLTALEM VKEVKEFAAC PEHKTSDSTF LVFMSHGIQE GICGTTYSNE VSDILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKDSVRD SEEDFLTDAI FEDDGIKKAH IEKDFIAFCS STPDNVSWRH PVRGSLFIES LIKHMKEYAW SCDLEDIFRK VRFSFEQPEF RLQMPTADRV TLTKRFYLFP GH
氨基酸119 to 402
标签His tag N-Terminus
额外的序列信息Large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. This protein contains about 284 aa without propeptide.
Our Abpromise guarantee covers the use of ab52079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 1181 Caspase-1, mouse recombinant. 1181-100 is the same size as the 100 unit size of ab52079.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 15% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70oC. Avoid multiple freeze/thaw cycles as activity might decrease.
- CASP1 nirs variant 1
相关性Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52079 被引用在 2 文献中.
- Ye B & Lai L Yu Shi An Chang Fang Ameliorates TNBS-Induced Colitis in Mice by Reducing Inflammatory Response and Protecting the Intestinal Mucosal Barrier. Evid Based Complement Alternat Med 2021:8870901 (2021). PubMed: 34055024
- Tang Y et al. Structure-based discovery of CZL80, a caspase-1 inhibitor with therapeutic potential for febrile seizures and later enhanced epileptogenic susceptibility. Br J Pharmacol 177:3519-3534 (2020). PubMed: 32346861