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AB276949

重组小鼠ARMET/ARP蛋白(His tag)

Recombinant Mouse ARMET/ARP protein (His tag)

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Recombinant Mouse ARMET/ARP protein (His tag) is a Mouse Full Length protein, in the 1 to 179 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

查看别名

Armet, Manf, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein, Protein ARMET

1 Images
SDS-PAGE - Recombinant Mouse ARMET/ARP protein (His tag) (AB276949)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse ARMET/ARP protein (His tag) (AB276949)

SDS-PAGE analysis of ab276949

关键信息

纯度

>90% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

HEK 293 cells

标签

His tag C-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q9CXI5

不含动物源

No

不含载体蛋白

No

种属

Mouse

存储溶液

pH: 7.4 Constituents: PBS

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MWATRGLAVALALSVLPDSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIEEELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGEMCKGCAEKSDYIRKINELMPKYAPKAASARTDL","proteinLength":"Full Length","predictedMolecularWeight":"19.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":179,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9CXI5","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ARMET also known as Arp is a 19 kDa protein known for its role in cellular stress responses. It is alternatively named MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) in some studies. This protein is widely expressed including in the brain liver and pancreas reflecting its involvement across different cellular environments. ARMET/ARP interacts with the endoplasmic reticulum to address misfolded protein loads and support cell survival under stress.
Biological function summary

ARMET plays a significant role in maintaining cellular homeostasis especially under stress conditions. It does not form a larger complex but functions as a standalone factor that aids in the proper folding of proteins within the endoplasmic reticulum. Additionally ARMET has protective effects on dopaminergic neurons which are critical for neuromolecular processes. This protein has connections to cellular survival pathways acting as a neurotrophic factor to support neuron maintenance and health.

Pathways

ARMET is an important player in the unfolded protein response (UPR) and the neuroprotective pathways. In these pathways ARMET interacts with other proteins involved in protein folding and stress responses such as BiP/GRP78. These interactions help prevent apoptosis and promote cell survival by ensuring that cells can cope with endoplasmic reticulum stress. The ability of ARMET to enhance neuronal survival links it to pathways important for neuroprotection.

ARMET has been associated with neurodegenerative diseases such as Parkinson's disease and diabetes. In Parkinson's disease the survival-promoting effects of ARMET on dopaminergic neurons become vital potentially offering therapeutic targets. Connections between ARMET and diabetes surface through its regulation of beta-cell survival in the pancreas suggesting a role in maintaining insulin production and glucose homeostasis. In these diseases ARMET may interact with other proteins like proinsulin which holds significance for maintaining endocrine functions.
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特殊说明

形式

Lyophilized

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常规信息

功能

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Stabilizes HSPA5/BiP in its substrate-bound ADP state, which facilitates HSPA5/BiP incorporation into chaperone-client complexes during endoplasmic reticulum stress, its interaction with HSPA5/BiP inhibits ATP binding to HSPA5/BiP and subsequent nucleotide exchange (PubMed : 30710085). As a result acts as a repressor of the unfolded protein response (UPR) pathway (PubMed : 30710085). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions. Essential for embryonic lung development (PubMed : 30543055). Required for the correct postnatal temporal and structural development of splenic white pulp (PubMed : 38159526). Required for the repair-associated myeloid response in skeletal muscle, acts as a regulator of phenotypic transition towards prorepair macrophages in response to muscle injury and as a result limits excessive proinflammatory signaling (PubMed : 37118549). Represses RELA expression and therefore NF-kB signaling in the myocardium, as a result limits macrophage infiltration of injured tissue and M1 macrophage differentiation in response to myocardial injury (PubMed : 33939070). Required for endochondral ossification in long bones and the skull during postnatal development (PubMed : 30543055).

序列相似性

Belongs to the ARMET family.

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产品实验方案

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靶点信息

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Stabilizes HSPA5/BiP in its substrate-bound ADP state, which facilitates HSPA5/BiP incorporation into chaperone-client complexes during endoplasmic reticulum stress, its interaction with HSPA5/BiP inhibits ATP binding to HSPA5/BiP and subsequent nucleotide exchange (PubMed : 30710085). As a result acts as a repressor of the unfolded protein response (UPR) pathway (PubMed : 30710085). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions. Essential for embryonic lung development (PubMed : 30543055). Required for the correct postnatal temporal and structural development of splenic white pulp (PubMed : 38159526). Required for the repair-associated myeloid response in skeletal muscle, acts as a regulator of phenotypic transition towards prorepair macrophages in response to muscle injury and as a result limits excessive proinflammatory signaling (PubMed : 37118549). Represses RELA expression and therefore NF-kB signaling in the myocardium, as a result limits macrophage infiltration of injured tissue and M1 macrophage differentiation in response to myocardial injury (PubMed : 33939070). Required for endochondral ossification in long bones and the skull during postnatal development (PubMed : 30543055).
See full target information Manf
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搭配使用产品

Primary Antibodies

AB316936

Anti-ARMET/ARP antibody [EPR29115-74] - BSA and Azide free

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody [EPR29115-74] - BSA and Azide free (AB316936)

  • 0
  • 0
Primary Antibodies

AB200040

Anti-HAND2 antibody [EPR19451]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-HAND2 antibody [EPR19451] (AB200040)

  • 5
  • 2
Primary Antibodies

AB196622

Anti-HAND1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HAND1 antibody (AB196622)

  • 0
  • 1
Primary Antibodies

AB28364

Anti-CD31 antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

  • 4
  • 161

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