重组小鼠AHA1蛋白(ab111143)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, WB
描述
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产品名称
重组小鼠AHA1蛋白
参阅全部 AHA1 蛋白酶 -
生物活性
2uM ab111143 generated a 9-fold ATPase activation of 2uM Hsp90 (His-tagged Hsp90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees celcius (this is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
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纯度
> 90 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
预测分子量
38 kDa -
氨基酸
1 to 338 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab111143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Constituents: 0.002% DTT, 0.378% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Activator of 90 kDa heat shock protein ATPase homolog 1
- Activator of heat shock 90kDa protein ATPase homolog 1
- AHA 1
see all -
功能
Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking. -
组织特异性
Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta. -
序列相似性
Belongs to the AHA1 family. -
细胞定位
Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab111143 尚未被引用在任何文献中。