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AB199750

重组mCherry蛋白(His tag)

Recombinant mCherry protein (His tag)

4

(1 Review)

|

(2 Publications)

Recombinant mCherry protein (His tag) is a Full Length protein, in the 1 to 236 aa range with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE and HPLC. The predicted molecular weight of ab199750 protein is 28.8 kDa.

- Save time and ensure accurate results - use our mCherry protein (His tag) protein as a control

关键信息

纯度

>97% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

HPLC, SDS-PAGE

applications

生物活性

No

不含动物源

No

不含载体蛋白

No

复溶

Reconstitute at 1 mg/mL in water

存储溶液

pH: 7.4 Constituents: 10.269% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Ensure the validity of your result using our recombinant mCherry protein (His tag) protein ab199750 as a control in SDS-PAGE.

Function

mCherry is the second generation monomeric red fluorescent protein that has improved brightness and photostability. The protein is suitable as a positive control for mCherry protein expression studies or as a labeling reagent. The recombinant mCherry protein ab199750 is ideal for fusion tag applications and is perfect for triple labeling with EGFP, CFP, YFP, or any other dyes.

The protein is engineered with 6xHis-tag on the N-terminus, which can be used for detection with anti-His-Tag antibody or protein purification/removal by using Ni++ beads.


Endotoxin level: <0.1 ng/µg


Check out our protein gel staining guide for SDS-PAGE here


This product is manufactured by BioVision, an Abcam company and was previously called 4993 mCherry Fluorescent Protein. 4993-100 is the same size as the 100 µg size of ab199750.

序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK","proteinLength":"Full Length","predictedMolecularWeight":"28.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":236,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[{"tag":"His","terminus":"N-Terminus"}]}]

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
False

特殊说明

形式

Lyophilized

附加说明

Greater than or equal to 97% by HPLC.

常规信息

产品实验方案

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 12: PubMed39591176

2024

Vaccination Against Androgen Receptor Splice Variants to Immunologically Target Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Robert D Marek,Selena Halabi,Mu-En Wang,Jason McBane,Junping Wei,Tao Wang,Xiao Yang,Congxiao Liu,Gangjun Lei,Herbert Kim Lyerly,Ming Chen,Timothy N Trotter,Zachary C Hartman

Biochemical and biophysical research communication 516:914-920 PubMed31272717

2019

Inhibition of allogeneic islet graft rejection by VISTA-conjugated liposome.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Guo,Shu Han,Yanfang Liu,Wenyuan Guo,Yuanyu Zhao,Fang Liu,Xiaomin Shi,Guoshan Ding,Quanxing Wang
View all publications

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