重组人/Murine/大鼠BDNF蛋白(Active) (ab9794)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Cellular Activation, SDS-PAGE, WB
描述
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产品名称
重组人/Murine/大鼠BDNF蛋白(Active)
参阅全部 BDNF 蛋白酶 -
生物活性
Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.
The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.
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纯度
> 98 % SDS-PAGE.
> 98% pure HPLC analyses. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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序列
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR -
预测分子量
27 kDa -
氨基酸
129 to 247 -
额外的序列信息
aa 129 to 247 refers to the full length mature form. This product does not contain signal peptide or propeptide.
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技术指标
Our Abpromise guarantee covers the use of ab9794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
HPLC
Cellular Activation
SDS-PAGE
Western blot
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.
常规信息
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别名
- Abrineurin
- ANON2
- BDNF
see all -
功能
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. -
组织特异性
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. -
疾病相关
Bulimia nervosa 2
Congenital central hypoventilation syndrome -
序列相似性
Belongs to the NGF-beta family. -
翻译后修饰
The propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). -
细胞定位
Secreted. - Information by UniProt
图片
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Rabbit Anti-BDNF antibody at 1 µg/ml +
Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 4 minutes
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (19)
ab9794 被引用在 19 文献中.
- Mazo CE et al. High Intensity Acute Aerobic Exercise Elicits Alterations in Circulating and Skeletal Muscle Tissue Expression of Neuroprotective Exerkines. Brain Plast 8:5-18 (2022). PubMed: 36448040
- Zloh M et al. High-Contrast Stimulation Potentiates the Neurotrophic Properties of Müller Cells and Suppresses Their Pro-Inflammatory Phenotype. Int J Mol Sci 23:N/A (2022). PubMed: 35955747
- Kim HJ et al. Brain-Derived Neurotrophic Factor Secreting Human Mesenchymal Stem Cells Improve Outcomes in Rett Syndrome Mouse Models. Front Neurosci 15:725398 (2021). PubMed: 34690674
- Kalinichenko SG et al. Brain-Derived Neurotrophic Factor (BDNF) As a Regulator of Apoptosis under Conditions of Focal Experimental Stroke. Bull Exp Biol Med 169:701-706 (2020). PubMed: 32990850
- Klimovich P et al. Three-Dimensional Model of Dorsal Root Ganglion Explant as a Method of Studying Neurotrophic Factors in Regenerative Medicine. Biomedicines 8:N/A (2020). PubMed: 32138155