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AB159886

重组人 ZP2 蛋白 (GST tag N-Terminus)

Recombinant Human ZP2 protein (GST tag N-Terminus)

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Recombinant Human ZP2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 736 aa range, expressed in Wheat germ, suitable for ELISA, WB.

查看别名

ZPA, ZP2, Zona pellucida sperm-binding protein 2, Zona pellucida glycoprotein 2, Zona pellucida protein A, Zp-2

1 Images
SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

ab159886 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, WB

applications

生物活性

No

访问

Q05996

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSGNSIDVSQLVNPAFPGTVTCDEREITVEFPSSPGTKKWHASVVDPLGLDMPNCTYILDPEKLTLRATYDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPAMQVEETQGLSASTICQKDFMSFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLPEAMKEGFSLLIDNHRMTFHVPFNATGVTHYVQGNSHLYMVSLKLTFISPGQKVIFSSQAICAPDPVTCNATHMTLTIPEFPGKLKSVSFENQNIDVSQLHDNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQFYLASLKLTFLLRPETVSMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALDLGTLRVGNSSCQPVFEAQSQGLVRFHIPLNGCGTRYKFEDDKVVYENEIHALWTDFPPSKISRDSEFRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPYGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWNVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":736,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q05996","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ZP2 also known as zona pellucida glycoprotein 2 is a structural protein critical to the protective coat surrounding oocytes called the zona pellucida. The protein is approximately 68 kDa in mass. ZP2 is highly expressed in the ovary. This glycoprotein specifically contributes to the matrix of the zona pellucida with other related proteins forming a structure that enables various reproductive events like sperm binding and prevention of polyspermy.
Biological function summary

ZP2 interacts with other zona pellucida proteins particularly ZP1 and ZP3 to form the zona pellucida matrix. This matrix allows the binding of sperm to the oocyte facilitating fertilization. The stability and integrity of this structure determine reproductive success and embryo development. ZP2 undergoes proteolytic cleavage during the fertilization process which is an important event for proper egg-sperm interaction.

Pathways

ZP2 forms an integral part of the fertilization pathway where it directly interacts with proteins ZP1 and ZP3. These proteins create a framework critical for sperm recognition and binding. Once ZP2 undergoes cleavage it assists in the progression of the acrosome reaction an important biological pathway for sperm penetration of the zona pellucida. The interactions and modifications of ZP2 within these pathways highlight the importance of precise regulation in reproduction.

Mutations or abnormalities in ZP2 are linked to reproductive conditions such as infertility. These changes can lead to disrupted zona pellucida integrity which affects sperm binding and fertilization ability. Additionally abnormalities in ZP2 relate to oocyte maturation defects impacting reproductive health. The protein also shows interplay with ZP3 in these conditions underlining its collaborative role in maintaining fertility.
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特殊说明

形式

Liquid

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常规信息

功能

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy (PubMed : 29895852). The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor (PubMed : 29895852).

序列相似性

Belongs to the ZP domain family. ZPA subfamily.

翻译后修饰

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.. Proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos.. N-glycosylated.. O-glycosylated; contains sulfate-substituted glycans.

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产品实验方案

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靶点信息

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy (PubMed : 29895852). The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor (PubMed : 29895852).
See full target information ZP2
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搭配使用产品

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

  • 0
  • 0
Primary Antibodies

AB250929

Anti-ZP2 antibody [EPR15467] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-ZP2 antibody [EPR15467] - BSA and Azide free (AB250929)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB162411

Recombinant Human MSY2/YBOX2/YBX2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human MSY2/YBOX2/YBX2 protein (GST tag N-Terminus) (AB162411)

  • 0
  • 0
Proteins & Peptides

AB159808

Recombinant Human ZAN protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZAN protein (GST tag N-Terminus) (AB159808)

  • 0
  • 0

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