重组人ZCH11蛋白(Tagged) (ab271776)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人ZCH11蛋白(Tagged)
参阅全部 ZCH11 蛋白酶 -
纯度
>= 90 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
SQKQQTCTDN TGDSDDSASG IEDVSDDLSK MKNDESNKEN SSEMDYLENA TVIDESALTP EQRLGLKQAE ERLERDHIFR LEKRSPEYTN CRYLCKLCLI HIENIQGAHK HIKEKRHKKN ILEKQEESEL RSLPPPSPAH LAALSVAVIE LAKEHGITDD DLRVRQEIVE EMSKVITTFL PECSLRLYGS SLTRFALKSS DVNIDIKFPP KMNHPDLLIK VLGILKKNVL YVDVESDFHA KVPVVVCRDR KSGLLCRVSA GNDMACLTTD LLTALGKIEP VFIPLVLAFR YWAKLCYIDS QTDGGIPSYC FALMVMFFLQ QRKPPLLPCL LGSWIEGFDP KRMDDFQLKG IVEEKFVKWE CNSSSATEKN SIAEENKAKA DQPKDDTKKT ETDNQSNAMK EKHGKSPLAL ETPNRVSLGQ LWLELLKFYT LDFALEEYVI CVRIQDILTR ENKNWPKRRI AIEDPFSVKR NVARSLNSQL VYEYVVERFR AAYRYFACPQ TKGGNKSTVD FKKREKGKIS NKKPVKSNNM ATNGCILLGE TTEKINAERE QPVQCDEMDC TSQRCIIDNN NLLVNELDFA DHGQDSSSLS TSKSSEIEPK LDKKQDDLAP SETCLKKELS QCNCIDLSKS PDPDKSTGTD CRSNLETESS HQSVCTDTSA TSCNCKATED ASDLNDDDNL PTQELYYVFD KFILTSGKPP TIVCSICKKD GHSKNDCPED FRKIDLKPLP PMTNRFREIL DLVCKRCFDE LSPPCSEQHN REQILIGLEK FIQKEYDEKA RLCLFGSSKN GFGFRDSDLD ICMTLEGHEN AEKLNCKEII ENLAKILKRH PGLRNILPIT TAKVPIVKFE HRRSGLEGDI SLYNTLAQHN TRMLATYAAI DPRVQYLGYT MKVFAKRCDI GDASRGSLSS YAYILMVLYF LQQRKPPVIP VLQEIFDGKQ IPQRMVDGWN AFFFDKTEEL KKRLPSLGKN TESLGELWLG LLRFYTEEFD FKEYVISIRQ KKLLTTFEKQ WTSKCIAIED PFDLNHNLGA GVSRKMTNFI MKAFINGRKL FGTPFYPLIG REAEYFFDSR VLTDGELAPN DRCCRVCGKI GHYMKDCPKR KS -
预测分子量
127 kDa including tags -
氨基酸
212 to 1313 -
标签
DDDDK tag C-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab271776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
100 ug/mL DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- DKFZp779C1943
- EC 2.7.7.52
- FLJ42878
see all -
功能
Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. -
序列相似性
Belongs to the DNA polymerase type-B-like family.
Contains 3 CCHC-type zinc fingers.
Contains 2 PAP-associated domains. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human ZCH11 protein (Tagged) (ab271776)
SDS-PAGE analysis of ab271776.
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Functional Studies - Recombinant Human ZCH11 protein (Tagged) (ab271776)Functional studies of ab271776.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271776 尚未被引用在任何文献中。