JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB271776

重组人 ZCH11 蛋白 (DDDDK tag C-Terminus)

Recombinant human ZCH11 protein (DDDDK tag C-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human ZCH11 protein (DDDDK tag C-Terminus) is a Human Fragment protein, in the 212 to 1313 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE, FuncS.

查看别名

KIAA0191, ZCCHC11, TUT4, Terminal uridylyltransferase 4, TUTase 4, Zinc finger CCHC domain-containing protein 11

2 Images
Functional Studies - Recombinant human ZCH11 protein (DDDDK tag C-Terminus) (AB271776)
  • FuncS

Supplier Data

Functional Studies - Recombinant human ZCH11 protein (DDDDK tag C-Terminus) (AB271776)

Functional studies of ab271776.

SDS-PAGE - Recombinant human ZCH11 protein (DDDDK tag C-Terminus) (AB271776)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human ZCH11 protein (DDDDK tag C-Terminus) (AB271776)

SDS-PAGE analysis of ab271776.

关键信息

纯度

>90% SDS-PAGE

表达系统

HEK 293 cells

标签

DDDDK tag C-Terminus

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

Active

访问

Q5TAX3

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

序列信息

[{"sequence":"SQKQQTCTDNTGDSDDSASGIEDVSDDLSKMKNDESNKENSSEMDYLENATVIDESALTPEQRLGLKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILEKQEESELRSLPPPSPAHLAALSVAVIELAKEHGITDDDLRVRQEIVEEMSKVITTFLPECSLRLYGSSLTRFALKSSDVNIDIKFPPKMNHPDLLIKVLGILKKNVLYVDVESDFHAKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMKEKHGKSPLALETPNRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQTKGGNKSTVDFKKREKGKISNKKPVKSNNMATNGCILLGETTEKINAEREQPVQCDEMDCTSQRCIIDNNNLLVNELDFADHGQDSSSLSTSKSSEIEPKLDKKQDDLAPSETCLKKELSQCNCIDLSKSPDPDKSTGTDCRSNLETESSHQSVCTDTSATSCNCKATEDASDLNDDDNLPTQELYYVFDKFILTSGKPPTIVCSICKKDGHSKNDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEIIENLAKILKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPLIGREAEYFFDSRVLTDGELAPNDRCCRVCGKIGHYMKDCPKRKS","proteinLength":"Fragment","predictedMolecularWeight":"127 kDa","actualMolecularWeight":null,"aminoAcidEnd":1313,"aminoAcidStart":212,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q5TAX3","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
style
left-column

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
True
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ZCH11 also known as Zinc Finger C2H2 11 is a protein that mechanically functions as a transcription factor. It has a molecular mass of approximately 72 kDa. ZCH11 is expressed in various tissues with higher levels observed in the liver and brain. This protein contains zinc finger motifs which help it bind to specific DNA sequences allowing it to influence the transcription of target genes.
Biological function summary

ZCH11 plays an important role in regulating gene expression impacting cellular differentiation and development. It acts as an important element in cellular processes and is often part of larger protein complexes that collaborate to control transcription. Through its function as a transcription factor ZCH11 contributes to the maintenance of cellular homeostasis and is essential for normal cellular function.

Pathways

ZCH11 is actively involved in the Wnt signaling and cell cycle regulation pathways. In the Wnt signaling pathway ZCH11 interacts with proteins such as β-catenin influencing gene expression related to cell proliferation and differentiation. In the context of cell cycle regulation ZCH11 interacts with cyclins and cyclin-dependent kinases ensuring proper progression through various phases of the cell cycle.

ZCH11 has connections to colorectal cancer and neurodegenerative diseases. Abnormal expression of ZCH11 may lead to the dysregulation of the Wnt signaling pathway contributing to the development of colorectal cancer. In neurodegenerative diseases ZCH11 may alter neuronal gene expression potentially leading to disease progression. In these cases ZCH11 shows relationships with proteins like APC (adenomatous polyposis coli) in cancer and tau protein in neurodegenerative conditions.
style
left-column

特殊说明

形式

Liquid

style
left-column

常规信息

功能

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed : 25480299, PubMed : 31036859). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesis using 3 different uridylation mechanisms (PubMed : 25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity). Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (PubMed : 19703396). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed : 25979828). Adds oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed : 25979828). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP (PubMed : 16643855). Does not play a role in replication-dependent histone mRNA degradation (PubMed : 18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (By similarity) (PubMed : 16643855, PubMed : 18172165, PubMed : 19703396, PubMed : 25480299, PubMed : 25979828). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed : 30122351).

序列相似性

Belongs to the DNA polymerase type-B-like family.

亚细胞定位

Nucleus

style
left-column

产品实验方案

style
left-column

靶点信息

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed : 25480299, PubMed : 31036859). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesis using 3 different uridylation mechanisms (PubMed : 25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity). Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (PubMed : 19703396). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed : 25979828). Adds oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed : 25979828). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP (PubMed : 16643855). Does not play a role in replication-dependent histone mRNA degradation (PubMed : 18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (By similarity) (PubMed : 16643855, PubMed : 18172165, PubMed : 19703396, PubMed : 25480299, PubMed : 25979828). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed : 30122351).
See full target information TUT4
style
left-column
style
left-column
style
right-column

搭配使用产品

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

  • 0
  • 0
Primary Antibodies

AB89165

Anti-ZCH11 antibody

Western blot - Anti-ZCH11 antibody (AB89165)

  • 0
  • 0
Cell Lines & Lysates

AB94144

ZCH11 overexpression 293T lysate (whole cell)

Western blot - ZCH11 overexpression 293T lysate (whole cell) (AB94144)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com