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重组人ZAP70蛋白(ab60963)

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SDS-PAGE - Recombinant human ZAP70 protein (ab60963)
  • Functional Studies - Recombinant human ZAP70 protein (ab60963)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 95% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies

描述

  • 产品名称

    重组人ZAP70蛋白
    参阅全部 ZAP70 蛋白酶

  • 纯度

    > 95 % Densitometry.
    Affinity purified.

  • 表达系统

    Baculovirus infected Sf9 cells

  • Accession

    P43403

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 预测分子量

      96 kDa including tags

    • 氨基酸

      1 to 619

    • 标签

      GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab60963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

  • 形式

    Liquid

  • 补充说明

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名

    • 70 kDa zeta associated protein
    • 70 kDa zeta-associated protein
    • EC 2.7.10.2
    • FLJ17670
    • FLJ17679
    • Selective T cell defect
    • SRK
    • STD
    • Syk related tyrosine kinase
    • Syk-related tyrosine kinase
    • Truncated ZAP kinase
    • Tyrosine protein kinase ZAP70
    • Tyrosine-protein kinase ZAP-70
    • TZK
    • ZAP 70
    • ZAP70
    • ZAP70_HUMAN
    • Zeta chain associated protein kinase 70kD
    • Zeta chain associated protein kinase 70kDa
    • Zeta chain associated protein kinase 70kDa isoform 1
    • Zeta chain associated protein kinase 70kDa isoform 2
    • Zeta chain of T cell receptor associated protein kinase 70
    • Zeta chain TCR associated protein kinase 70kD
    • Zeta chain TCR associated protein kinase 70kDa
    see all

  • 功能

    Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

  • 组织特异性

    Expressed in T- and natural killer cells.

  • 疾病相关

    Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.

  • 结构域

    The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

  • 翻译后修饰

    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

  • 细胞定位

    Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt

图片

  • SDS-PAGE - Recombinant human ZAP70 protein (ab60963)
    SDS-PAGE - Recombinant human ZAP70 protein (ab60963)
    SDS PAGE analysis of ab60963
  • Functional Studies - Recombinant human ZAP70 protein (ab60963)
    Functional Studies - Recombinant human ZAP70 protein (ab60963)
    The specific activity of ZAP70 (ab60963) was determined to be 129 nmol/min/mg as per activity assay protocol

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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