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AB198661

重组人WTAP蛋白

Recombinant Human WTAP protein

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(0 Publication)

Recombinant Human WTAP protein is a Human Full Length protein, in the 1 to 151 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

KIAA0105, WTAP, Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, WT1-associated protein, Wilms tumor 1-associating protein, hFL(2)D

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

T7 tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q15007

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MASMTGGQQMGRGEFMTNEEPLPKKVRLSETDFKVMARDELILRWKQYEAYVQALEGKYTDLNSNDVTGLRESEEKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDR","proteinLength":"Full Length","predictedMolecularWeight":"19.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":151,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15007","tags":[{"tag":"T7","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

WTAP also known as Wilms tumor 1-associated protein is a core component of the writer complex that catalyzes N6-methyladenosine (m6A) methylation on mRNA. With a molecular mass of approximately 39 kDa WTAP localizes almost ubiquitously in human tissues but shows higher expression in liver and kidney cells. This protein assists in the regulation of mRNA splicing and stability functioning as an adapter for RNA binding proteins.
Biological function summary

This protein plays a critical role in post-transcriptional gene regulation by being part of the m6A methyltransferase complex which includes other important proteins like METTL3 and METTL14. WTAP does not bind RNA directly but helps its partners achieve target specificity during gene expression processes. The presence of WTAP within the complex stabilizes and coordinates the activity of the entire methyltransferase assembly.

Pathways

The m6A methylation WTAP facilitates affects various pathways including mRNA splicing and degradation. This process intricately connects WTAP to the regulation of the JAK-STAT signaling pathway where its activity influences inflammatory responses and cell proliferation. Additionally WTAP has ties to the Wnt/β-catenin pathway collaborating with proteins like METTL3 to regulate gene expression involved in cell fate determination and embryogenesis.

Research shows WTAP has connections to various cancers such as hepatocellular carcinoma due to its role in mRNA processing and stability. This association often involves its interaction with other oncogenic proteins like METTL3 leading to aberrant gene expression patterns. WTAP also has relevance in acute myeloid leukemia where it influences mRNA maturation and impacts cell differentiation and proliferation.
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特殊说明

形式

Liquid

附加说明

ab198661 was expressed in E.coli as inclusion bodies, refolded using unique “temperature shift inclusion body refolding” technology, chromatographically purified and sterile filtered.

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常规信息

功能

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed : 29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed : 24316715, PubMed : 24407421, PubMed : 24981863). Acts as a mRNA splicing regulator (PubMed : 12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed : 17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed : 17095724).

序列相似性

Belongs to the fl(2)d family.

亚细胞定位

Nucleus speckle

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产品实验方案

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靶点信息

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed : 29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed : 24316715, PubMed : 24407421, PubMed : 24981863). Acts as a mRNA splicing regulator (PubMed : 12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed : 17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed : 17095724).
See full target information WTAP
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搭配使用产品

Proteins & Peptides

AB271611

Recombinant Human METTL3 protein (Tagged)

Western blot - Recombinant Human METTL3 protein (Tagged) (AB271611)

  • 0
  • 0
Primary Antibodies

AB195380

Anti-WTAP antibody [EPR18744]

RabMAb|Recombinant|KO Validated

Western blot - Anti-WTAP antibody [EPR18744] (AB195380)

  • 5
  • 6
Proteins & Peptides

AB268789

Recombinant human METTL3 + METTL14 protein (Active)

Functional Studies - Recombinant human METTL3 + METTL14 protein (Active) (AB268789)

  • 0
  • 0
Primary Antibodies

AB195352

Anti-METTL3 antibody [EPR18810]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry - Anti-METTL3 antibody [EPR18810] (AB195352)

  • 5
  • 8

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