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AB204627

Recombinant Human Wnt5a protein

Recombinant Human Wnt5a protein

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(4 Publications)

Recombinant Human Wnt5a protein is a Human Full Length protein, in the 62 to 380 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

查看别名

Protein Wnt-5a, WNT5A

1 Images
SDS-PAGE - Recombinant Human Wnt5a protein (AB204627)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Wnt5a protein (AB204627)

15% SDS-PAGE analysis of ab204627 under reducing condtions and stained with Coomassie blue.

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

His tag C-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

P41221

不含动物源

No

不含载体蛋白

No

种属

Human

复溶

Reconstitute at 0.1 mg/mL in water

存储溶液

Constituents: 0.3% Acetic acid

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

No direct biological data available at the moment but ab204627 binds to immobilized Human sROR2 in a functional ELISA.
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序列信息

[{"sequence":"MIIGAQPLCSQLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTVDNTSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSTCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKCTEIVDQFVCKLEHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"36.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":380,"aminoAcidStart":62,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P41221","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Wnt5a also known simply as Wnt-5a is a member of the Wnt family of signaling proteins. It plays a role in non-canonical Wnt signaling which means it does not involve the typical Wnt/beta-catenin pathway. The protein has a molecular weight of approximately 42 kDa and is widely expressed in various tissues including the brain lungs and reproductive organs. Wnt5a influences several cellular processes such as cell movement polarity and organ development by binding to Frizzled family receptors and co-receptors like ROR1 and ROR2.
Biological function summary

Wnt5a drives critical processes in development and tissue homeostasis. It acts by guiding cell migration and establishing cell polarity. Wnt5a is not part of a single large complex but interacts with membrane receptors and co-receptors to exert its effects. This interaction initiates signaling cascades that regulate cytoskeletal dynamics which is essential in both embryonic development and adult tissue maintenance. Wnt5a's role is pivotal in maintaining cellular organization and function across different biological contexts.

Pathways

Wnt5a participates significantly in the planar cell polarity pathway and the Wnt/Ca2+ signaling pathway. In these pathways Wnt5a affects the activity of proteins such as Dishevelled and CaMKII to influence cellular functions independently from beta-catenin. These pathways regulate processes like tissue morphogenesis and cell orientation maintaining the spatial arrangement and structural integrity of tissues.

Wnt5a has been linked to cancer progression and inflammatory diseases such as rheumatoid arthritis. It interacts with proteins like ROR1 and ROR2 during these pathologies. In cancer abnormal Wnt5a signaling can lead to enhanced cell migration and invasion contributing to metastasis. In rheumatoid arthritis Wnt5a may drive inflammatory pathways and joint tissue damage. Researchers consider Wnt5a a target for therapeutic interventions in these conditions with ongoing studies focused on modulating its activity for disease management.
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特殊说明

形式

Lyophilized

附加说明

Purified by Nickel Sepharose HighTrap GEEndotoxin level: < 0.1 ng per μg of Wnt5a

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常规信息

功能

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed : 15735754). Mediates motility of melanoma cells (PubMed : 17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).

序列相似性

Belongs to the Wnt family.

翻译后修饰

Glycosylation is necessary for secretion but not for activity.. Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.. Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A.

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产品实验方案

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靶点信息

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed : 15735754). Mediates motility of melanoma cells (PubMed : 17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).
See full target information WNT5A
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111511 PubMed36261010

2022

A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin Ghochani,Sree Deepthi Muthukrishnan,Alireza Sohrabi,Riki Kawaguchi,Michael C Condro,Soniya Bastola,Fuying Gao,Yue Qin,Jack Mottahedeh,M Luisa Iruela-Arispe,Nagesh Rao,Dan R Laks,Linda M Liau,Gary W Mathern,Steven A Goldman,S Thomas Carmichael,Ichiro Nakano,Giovanni Coppola,Stephanie K Seidlits,Harley I Kornblum

Stem cells international 2022:9124277 PubMed35126528

2022

Scrapie-Responsive Gene 1 Promotes Chondrogenic Differentiation of Umbilical Cord Mesenchymal Stem Cells via Wnt5a.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchuan Zhou,Tian Zheng,Lin Li,Yangfang Guo,Jiayu Xiao,Jin Wang,Zhiqin Du,Hui Gao,Weiwei Tang,Liusan Yang,Haiyan Hu,Xiaodan Wang,Mingyao Meng,Zongliu Hou

Cell reports 33:108472 PubMed33296652

2020

Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoning Wang,Miao Cui,Akansha M Shah,Wei Tan,Ning Liu,Rhonda Bassel-Duby,Eric N Olson

Journal of integrative neuroscience 18:309-312 PubMed31601081

2019

The Wnt/β-catenin signaling pathway affects the distribution of cytoskeletal proteins in Aβ treated PC12 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifeng Tian,Xiaoling Zhang,Zhijun Zhao,Fuhua Zhang,Tongxing Deng
View all publications
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搭配使用产品

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB227229

Anti-Wnt5a antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (AB227229)

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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Proteins & Peptides

AB81484

Recombinant mouse Wnt3a protein

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