重组人Wnt2b蛋白(ab132538)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: WB, ELISA, SDS-PAGE
描述
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产品名称
重组人Wnt2b蛋白
参阅全部 Wnt2b 蛋白酶 -
表达系统
Wheat germ -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MLRPGGAEEAAQLPLRRASAPVPVPSPAAPDGSRASARLGLACLLLLLLL TLPARVDTSWWYIGALGARVICDNIPGLVSRQRQLCQRYPDIMRSVGEGA REWIRECQHQFRHHRWNCTTLDRDHTVFGRVMLRSSREAAFVYAISSAGV VHAITRACSQGELSVCSCDPYTRGRHHDQRGDFDWGGCSDNIHYGVRFAK AFVDAKEKRLKDARALMNLHNNRCGRTAVRRFLKLECKCHGVSGSCTLRT CWRALSDFRRTGDYLRRRYDGAVQVMATQDGANFTAARQGYRRATRTDLV YFDNSPDYCVLDKAAGSLGTAGRVCSKTSKGTDGCEIMCCGRGYDTTRVT RVTQCECKFHWCCAVRCKECRNTVDVHTCKAPKKAEWLDQT -
预测分子量
70 kDa including tags -
氨基酸
1 to 391 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab132538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
ELISA
SDS-PAGE
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形式
Liquid -
补充说明
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
常规信息
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别名
- Protein Wnt-13
- Protein Wnt-2b
- Wingless type MMTV integration site family, member 13
see all -
功能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis. -
组织特异性
Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines. -
序列相似性
Belongs to the Wnt family. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human Wnt2b protein (ab132538)12.5% SDS-PAGE Stained with Coomassie Blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab132538 被引用在 2 文献中.
- Ross ADB et al. Obtaining purified human intestinal epithelia for single-cell analysis and organoid culture. STAR Protoc 2:100597 (2021). PubMed: 34169291
- Elmentaite R et al. Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn's Disease. Dev Cell 55:771-783.e5 (2020). PubMed: 33290721
