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AB269136

重组人WNK3蛋白(Active)

Recombinant human WNK3 protein (Active)

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Recombinant human WNK3 protein (Active) is a Human Fragment protein, in the 1 to 571 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

查看别名

KIAA1566, PRKWNK3, WNK3, Serine/threonine-protein kinase WNK3, Protein kinase lysine-deficient 3, Protein kinase with no lysine 3

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Functional Studies - Recombinant human WNK3 protein (Active) (AB269136)
  • FuncS

Supplier Data

Functional Studies - Recombinant human WNK3 protein (Active) (AB269136)

ab269136 specific activity was determined to be 13 nmol/min/mg in a kinase assay using myelin basic protein (MBP) substrate.

SDS-PAGE - Recombinant human WNK3 protein (Active) (AB269136)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human WNK3 protein (Active) (AB269136)

SDS-PAGE analysis of ab269136.

关键信息

纯度

>90% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

ab269136 specific activity was determined to be 13 nmol/min/mg in a kinase assay using myelin basic protein (MBP) substrate.

访问

Q9BYP7

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEA","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":571,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9BYP7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

WNK3 also known as With No Lysine [K] kinase 3 is a serine/threonine-protein kinase weighing approximately 203 kDa. Its expression occurs in various tissues with notable presence in the kidney brain and liver. Structurally WNK3 contains several domains that interact with proteins involved in ion transport and signaling pathways. WNK3 executes its mechanical functions by influencing ion transporter activity such as by regulating the sodium-chloride cotransporter (NCC) and the sodium-potassium-chloride cotransporter (NKCC).
Biological function summary

WNK3 plays a critical role in cellular ion homeostasis and modulation of ion transporters in response to osmotic changes. It is part of a complex that regulates ion balance within cells affecting cell volume and blood pressure. Through phosphorylation activities WNK3 can activate or inhibit other kinases impacting cellular responses to various stimuli. These regulatory actions are significant for maintaining proper electrolyte balance and fluid dynamics in the body.

Pathways

WNK3 is involved in regulating the WNK-SPAK/OSR1 pathway which is essential in controlling ion transporter activities in cells. This pathway fine-tunes the activity of the NCC and NKCC transporters. WNK3 is closely connected with other kinases like WNK1 and WNK4 with all playing common roles in blood pressure regulation and ion transport. The coordination between these kinases ensures precise cellular responses to changes in osmolarity and extracellular signals.

WNK3's regulation of ion balance connects it to hypertension and congenital nephrologic disorders. Hypertension links to the dysregulation of electrolyte transport and blood pressure control influenced by WNK3. Furthermore mutations in WNK3 alongside related proteins like WNK1 and NCC can result in altered renal salt handling leading to congenital disorders such as pseudohypoaldosteronism type II. Understanding WNK3's function and interactions offers valuable insights into treatments targeting these conditions.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Serine/threonine-protein kinase component of the WNK3-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress (PubMed : 16275911, PubMed : 16275913, PubMed : 16501604, PubMed : 22989884, PubMed : 36318922). WNK3 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation (PubMed : 36318922). The membraneless compartment concentrates WNK3 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK3-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK (PubMed : 22989884). Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A4/KCC1, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed : 16275911, PubMed : 16275913). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A4/KCC1, SLC12A5/KCC2 and SLC12A6/KCC3 inhibits its activity, blocking ion efflux (PubMed : 16275911, PubMed : 16275913, PubMed : 16357011, PubMed : 19470686, PubMed : 21613606). Phosphorylates WNK4, possibly regulating the activity of SLC12A3/NCC (PubMed : 17975670). May also phosphorylate NEDD4L (PubMed : 20525693). Also acts as a scaffold protein independently of its protein kinase activity : negatively regulates cell membrane localization of various transporters and channels, such as KCNJ1 and SLC26A9 (PubMed : 16357011, PubMed : 17673510). Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed : 18768590).

序列相似性

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.

翻译后修饰

Autophosphorylated at Ser-304 and Ser-308, promoting its activity (PubMed:33439774). Phosphorylation at Thr-541 prevents interaction with KLHL3 and subsequent ubiquitination and degradation by the BCR(KLHL3) complex (Probable) (PubMed:35179207).. Ubiquitinated by the BCR(KLHL2) complex, leading to its degradation (PubMed:23838290). Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation (PubMed:35179207).

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产品实验方案

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靶点信息

Serine/threonine-protein kinase component of the WNK3-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress (PubMed : 16275911, PubMed : 16275913, PubMed : 16501604, PubMed : 22989884, PubMed : 36318922). WNK3 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation (PubMed : 36318922). The membraneless compartment concentrates WNK3 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK3-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK (PubMed : 22989884). Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A4/KCC1, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed : 16275911, PubMed : 16275913). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A4/KCC1, SLC12A5/KCC2 and SLC12A6/KCC3 inhibits its activity, blocking ion efflux (PubMed : 16275911, PubMed : 16275913, PubMed : 16357011, PubMed : 19470686, PubMed : 21613606). Phosphorylates WNK4, possibly regulating the activity of SLC12A3/NCC (PubMed : 17975670). May also phosphorylate NEDD4L (PubMed : 20525693). Also acts as a scaffold protein independently of its protein kinase activity : negatively regulates cell membrane localization of various transporters and channels, such as KCNJ1 and SLC26A9 (PubMed : 16357011, PubMed : 17673510). Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed : 18768590).
See full target information WNK3
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搭配使用产品

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