Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

重组人WISP3蛋白(ab50049)

Submit a review Q&A (1)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

描述

  • 产品名称

    重组人WISP3蛋白

  • 生物活性

    Biological Activity : The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 µg/ml.

  • 纯度

    > 98 % SDS-PAGE.
    Greater than 98% by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).

  • 表达系统

    Escherichia coli

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 序列

      TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQPCD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFCGICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVCQRNCRE PGDIFSELKI L

相关产品

技术指标

Our Abpromise guarantee covers the use of ab50049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Functional Studies

  • 形式

    Lyophilized
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    For lot specific reconstitution information please contact our Scientific Support Team.

常规信息

  • 别名

    • CCN 6
    • CCN family member 6
    • CCN6
    • CYR61
    • LIBC
    • Lost in inflammatory breast cancer tumor suppressor protein
    • MGC125987
    • MGC125988
    • MGC125989
    • OTTHUMP00000040421
    • PPAC
    • PPD
    • UNQ462/PRO790/PRO956
    • WISP 3
    • WISP-3
    • WISP3
    • WISP3_HUMAN
    • WNT 1 inducible signaling pathway protein 3
    • Wnt 1 signaling pathway protein 3
    • WNT1 inducible signaling pathway protein 3
    • WNT1 inducible signaling pathway protein 3 precursor
    • WNT1-inducible-signaling pathway protein 3
    see all

  • 功能

    Appears to be required for normal postnatal skeletal growth and cartilage homeostasis.

  • 组织特异性

    Predominant expression in adult kidney and testis and fetal kidney. Weaker expression found in placenta, ovary, prostate and small intestine. Also expressed in skeletally-derived cells such as synoviocytes and articular cartilage chondrocytes.

  • 疾病相关

    Defects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC) [MIM:208230]. PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging.

  • 序列相似性

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.

  • 细胞定位

    Secreted.
  • Information by UniProt

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab50049？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab50049 尚未被引用在任何文献中。

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

Question

Please confirm if this has functional activity in cell culture (Functional studies listed as tested application)

Read More
Answer

Thank you for your telephone enquiry yesterday afternoon. I can confirm that our ab50049 WISP3 human protein (Active) has been tested for biological activity in cell culture, so it will be covered by our guarantee for this application. The originator of the protein has kindly provided the following information which I hope you will find helpful: The ED50 was determined by the dose-dependent proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 µg/ml. The protocol used was as follows: Note: As of lot 020405 culture and assay media have been changed to media containing no phenol red. Also the cells are cultured in 10% Certified FBS, but the assay media contains 0.25% HI-FBS. 1. Resuspend cells in assay media containing 0.25% HI-FBS Transfer 100ul culture/well to assay plate (5,000 cells/well; passage 22). 2. Serial dilute h-WISP-3 in assay media in duplicate. 3. Add 100ul/well of h-WISP-3 to cells in assay plate. Final assay volume is 200ul/well containing 0.25% HI-FBS, and h-WISP-3. 4. Incubate 90 hours. 5. Add 20ul of Substrate Cell Titer Aqueous One Solution Reagent to each well. 6. Incubate 37oC and read OC at 490nm. The endotoxin level was tested for this and was found to be less than 0.1 ng per µg (1EU/µg). I hope this information is useful to you. Please do not hesitate to contact us should you have any additional questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com