重组人WHSC2/NELF-A蛋白(ab116197)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人WHSC2/NELF-A蛋白 -
纯度
> 85 % SDS-PAGE.
ab116197 was purified using conventional chromatography techniques. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHHSSGLVPRGSHMPGQRRALSPKMASMRESDTGLWLHNKLGA TDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVD EMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQD ILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKP KSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPK QAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGR EAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPAL PSTSYLPSTPSVVPASSYIPSSETPPAPSSREASRPPEEPSAPSPTLPAQ FKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQT QAPAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSREN PCQEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFK KYKPMTNVS -
预测分子量
61 kDa including tags -
氨基酸
1 to 539 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab116197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
补充说明
Protein previously labeled as WHSC2.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.08% DTT, 0.32% Tris HCl, 0.03% EDTA, 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride
常规信息
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别名
- FLJ10442
- FLJ25112
- Negative elongation factor A
see all -
功能
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. -
组织特异性
Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney. -
序列相似性
Belongs to the NELF-A family.
Contains 1 HDAg-like domain. -
结构域
The HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex. -
细胞定位
Nucleus. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human WHSC2/NELF-A protein (ab116197)
15% SDS-PAGE analysis of 3 µg ab116197.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab116197 尚未被引用在任何文献中。