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AB198071

重组人 VPS34 蛋白 (DDDDK tag N-Terminus)

Recombinant human VPS34 protein (DDDDK tag N-Terminus)

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Recombinant human VPS34 protein (DDDDK tag N-Terminus) is a Human Full Length protein, in the 2 to 887 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

查看别名

VPS34, PIK3C3, Phosphatidylinositol 3-kinase catalytic subunit type 3, PI3-kinase type 3, PI3K type 3, PtdIns-3-kinase type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, hVps34

2 Images
Functional Studies - Recombinant human VPS34 protein (DDDDK tag N-Terminus) (AB198071)
  • FuncS

Supplier Data

Functional Studies - Recombinant human VPS34 protein (DDDDK tag N-Terminus) (AB198071)

Example of specific activity of ab198071.

SDS-PAGE - Recombinant human VPS34 protein (DDDDK tag N-Terminus) (AB198071)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human VPS34 protein (DDDDK tag N-Terminus) (AB198071)

10% SDS-PAGE analysis of ab198071 (4 μg) with Coomassie staining.

关键信息

纯度

>70% SDS-PAGE

Affinity purified.

表达系统

Baculovirus infected Sf9 cells

标签

DDDDK tag N-Terminus

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

Specific Activity: 140 pmol/min/μg.

访问

Q8NEB9

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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搭配使用产品

Proteins & Peptides

AB84769

Recombinant Human PI 3 Kinase p85 alpha protein

Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (AB84769)

  • 0
  • 0
Proteins & Peptides

AB159127

Recombinant Human PI 3 Kinase catalytic subunit alpha/PIK3CA protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human PI 3 Kinase catalytic subunit alpha/PIK3CA protein (GST tag N-Terminus) (AB159127)

  • 0
  • 0
Primary Antibodies

AB191606

Anti-PI 3 Kinase p85 alpha antibody [EPR18702]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (AB191606)

  • 5
  • 2
Primary Antibodies

AB124905

Anti-VPS34 antibody [EPR5301]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS34 antibody [EPR5301] (AB124905)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

This product was previously labelled as PI 3 Kinase Class 3

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序列信息

[{"linker":null,"sequence":"GEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALELLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK","proteinLength":"Full Length","predictedMolecularWeight":"98.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":887,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8NEB9","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
True
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常规信息

功能

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed : 14617358, PubMed : 33637724, PubMed : 7628435). As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding (PubMed : 32690950). Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123). Involved in the transport of lysosomal enzyme precursors to lysosomes (By similarity). Required for transport from early to late endosomes (By similarity).. (Microbial infection) Kinase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.

序列相似性

Belongs to the PI3/PI4-kinase family.

翻译后修饰

Ubiquitinated via 'Lys-29'- and 'Lys-48'-linked ubiquitination by UBE3C, promoting its degradation (PubMed:33637724). Deubiquitination by ZRANB1/TRABID promotes its stabilization, leading to autophagosome maturation (PubMed:33637724).

亚细胞定位

Late endosome

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产品实验方案

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靶点信息

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed : 14617358, PubMed : 33637724, PubMed : 7628435). As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding (PubMed : 32690950). Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123). Involved in the transport of lysosomal enzyme precursors to lysosomes (By similarity). Required for transport from early to late endosomes (By similarity).. (Microbial infection) Kinase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
See full target information PIK3C3
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