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重组人Vitamin D Receptor蛋白(ab82068)

Submit a review Q&A (2)
Western blot - Recombinant Human Vitamin D Receptor protein (ab82068)

    Key features and details

    • Expression system: Baculovirus infected insect cells
    • Purity: > 95% HPLC
    • Suitable for: GSA, WB

    描述

    • 产品名称

      重组人Vitamin D Receptor蛋白

    • 纯度

      > 95 % HPLC.
      Affinity purified to >95% purity.

    • 表达系统

      Baculovirus infected insect cells

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 氨基酸

        1 to 427

    相关产品

    技术指标

    Our Abpromise guarantee covers the use of ab82068 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Gel Supershift Assays

      Western blot

      EMSA

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.9
      Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)

    常规信息

    • 别名

      • 1
      • 1 25 dihydroxyvitamin D3 receptor
      • 1,25 dihydroxyvitamin D3 receptor
      • 1,25-@dihydroxyvitamin D3 receptor
      • 25-dihydroxyvitamin D3 receptor
      • Member 1
      • NR1I1
      • Nuclear receptor subfamily 1 group I member 1
      • PPP1R163
      • Protein phosphatase 1, regulatory subunit 163
      • VDR
      • VDR_HUMAN
      • Vitamin D (1,25- dihydroxyvitamin D3) receptor
      • Vitamin D hormone receptor
      • Vitamin D nuclear receptor variant 1
      • Vitamin D receptor
      • Vitamin D3 receptor
      see all

    • 功能

      Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.

    • 疾病相关

      Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.

    • 序列相似性

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • 结构域

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    图片

    • Western blot - Recombinant Human Vitamin D Receptor protein (ab82068)
      Western blot - Recombinant Human Vitamin D Receptor protein (ab82068)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab82068？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab82068 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Hi, I have a question about the activity of vitamin D receptor (ab82068). Could I use this VDR for competitive binding assay, e.g 1,25dihydroxyvitamin D3? Also, the concentration of VDR is pretty high in stock. Which buffer shall I use to dilute the VDR protein? and how is the stability of the diluted protein at -20 or -80? Thanks for answering these questions?

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    Answer

    Apologies for the delay in replying to your question. The unit size is 5 ug and the concentration 0.5 ug/ul, therefore you will receive 10 ul. We suggest diluting the protein in the storage buffer, then aliquot and freeze. The storage buffer is as follows: 20 mM Tris-Cl (pH 8.0) 20% Glycerol 100 mM KCl 1 mM DTT 0.2 mM EDTA. I hope this information helps. Please do not hesitate to contact me should you have further questions.

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    Question

    Hi, I have a question about the activity of vitamin D receptor (ab82068). Could I use this VDR for competitive binding assay, e.g 1,25dihydroxyvitamin D3? Also, the concentration of VDR is pretty high in stock. Which buffer shall I use to dilute the VDR protein? and how is the stability of the diluted protein at -20 or -80? Thanks for answering these questions?

    Read More
    Answer

    Our product, ab82068, can be used in competitive binding assays. This protein can be diluted in whatever buffer is appropriate for use in your assay; however I would like to point out that we do not recommend diluting this antibody before freezing it. I would recommend that you freeze the antibody in small aliquots and when you are ready to use the antibody in your application thaw it out and then dilute it.

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