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AB82068

重组人Vitamin D Receptor蛋白

Recombinant Human Vitamin D Receptor protein

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Recombinant Human Vitamin D Receptor protein is a Human Full Length protein, in the 1 to 427 aa range, expressed in Baculovirus, with >95%, suitable for GSA, EMSA, WB.

查看别名

NR1I1, VDR, Vitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1

1 Images
Western blot - Recombinant Human Vitamin D Receptor protein (AB82068)
  • WB

Unknown

Western blot - Recombinant Human Vitamin D Receptor protein (AB82068)

All lanes:

Anti-Vitamin D Receptor antibody (<a href='/products/unavailable/vitamin-d-receptor-antibody-ab82566'>ab82566</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Vitamin D Receptor protein (ab82068) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 20s

关键信息

纯度

>95% HPLC

表达系统

Baculovirus

标签

Tag free

应用

EMSA, GSA, WB

applications

生物活性

No

访问

P11473

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.9 Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "GSA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "EMSA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab82068 can be used as a WB positive control in conjunction with <a href='/products/unavailable/vitamin-d-receptor-antibody-ab82566'>ab82566</a>.</p>" } } }
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序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":427,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P11473","tags":[]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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特殊说明

形式

Liquid

附加说明

Affinity purified to >95% purity.

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常规信息

功能

Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells (PubMed : 10678179, PubMed : 15728261, PubMed : 16913708, PubMed : 28698609, PubMed : 37478846). Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR (PubMed : 28698609). The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes (PubMed : 28698609). Plays a central role in calcium homeostasis (By similarity). Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites (PubMed : 12016314, PubMed : 32354638).

序列相似性

Belongs to the nuclear hormone receptor family. NR1 subfamily.

翻译后修饰

Ubiquitinated by UBR5, leading to its degradation: UBR5 specifically recognizes and binds ligand-bound VDR when it is not associated with coactivators (NCOAs) (PubMed:37478846). In presence of NCOAs, the UBR5-degron is not accessible, preventing its ubiquitination and degradation (PubMed:37478846).

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells (PubMed : 10678179, PubMed : 15728261, PubMed : 16913708, PubMed : 28698609, PubMed : 37478846). Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR (PubMed : 28698609). The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes (PubMed : 28698609). Plays a central role in calcium homeostasis (By similarity). Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites (PubMed : 12016314, PubMed : 32354638).
See full target information VDR
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搭配使用产品

Cell Lines & Lysates

AB257796

Human VDR (Vitamin D Receptor) knockout HeLa cell lysate

Western blot - Human VDR (Vitamin D Receptor) knockout HeLa cell lysate (AB257796)

  • 0
  • 0
Primary Antibodies

AB109234

Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade

RabMAb|Recombinant|KO Validated

ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (AB109234)

  • 0
  • 0
Proteins & Peptides

AB256429

Recombinant Rubella Virus spike glycoprotein E1 protein (Fc Chimera)

Functional Studies - Recombinant Rubella Virus spike glycoprotein E1 protein (Fc Chimera) (AB256429)

  • 0
  • 0
Proteins & Peptides

AB198770

Recombinant Human UHRF1 protein

SDS-PAGE - Recombinant Human UHRF1 protein (AB198770)

  • 0
  • 0

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