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AB316517

重组人Visfatin蛋白Standard (His tag)

Recombinant Human Visfatin Protein Standard (His tag)

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Recombinant Human Visfatin Protein Standard (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, sELISA.

查看别名

PBEF, PBEF1, NAMPT, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor

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Sandwich ELISA - Recombinant Human Visfatin Protein Standard (His tag) (AB316517)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Visfatin Protein Standard (His tag) (AB316517)

Sandwich ELISA with the capture antibody ab267449 dilution at 2 μg/mL and detector antibody ab267450 dilution at 0.5 μg/mL after biotin conjugation.

SDS-PAGE - Recombinant Human Visfatin Protein Standard (His tag) (AB316517)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Visfatin Protein Standard (His tag) (AB316517)

SDS-PAGE analysis of ab316517 under reducing conditions for 2ug protein.

关键信息

纯度

>80% SDS-PAGE

表达系统

Escherichia coli

标签

His tag C-Terminus

应用

SDS-PAGE, sELISA

applications

生物活性

No

访问

P43490

不含动物源

Yes

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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  • 0
Proteins & Peptides

AB256228

Recombinant Human Chemerin protein

SDS-PAGE - Recombinant Human Chemerin protein (AB256228)

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  • 0
Primary Antibodies

AB236874

Anti-Visfatin antibody [EPR21980]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [EPR21980] (AB236874)

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  • 0
Primary Antibodies

AB72965

Anti-Chemerin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Chemerin antibody (AB72965)

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  • 1
Assay Kits

AB221819

NAMPT Activity Assay Kit (Colorimetric)

Functional Studies - NAMPT Activity Assay Kit (Colorimetric) (AB221819)

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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序列信息

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"57 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P43490","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification His Tag
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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常规信息

功能

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component : CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).

序列相似性

Belongs to the NAPRTase family.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component : CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
See full target information NAMPT
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