重组人Visfatin蛋白(ab50134)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
-
产品名称
重组人Visfatin蛋白
参阅全部 Visfatin 蛋白酶 -
生物活性
The ED50 was determined by the dose-dependent proliferation of the RPMI 8226 cells. The expected ED50 for this effect is 15.0-20.0 ng/ml.
-
纯度
> 95 % SDS-PAGE.
Purity: greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH
-
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab50134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
Functional Studies
-
形式
Lyophilized -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.036% Hydrochloric acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. Note: Due to solubility reasons the protein should be kept at low pH. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
常规信息
-
别名
- 1110035O14Rik
- AI314458
- AI480535
see all -
功能
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
组织特异性
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
通路
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
序列相似性
Belongs to the NAPRTase family. -
细胞定位
Cytoplasm. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab50134 尚未被引用在任何文献中。