重组人VEGFE蛋白(ab53679)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人VEGFE蛋白
参阅全部 VEGFE 蛋白酶 -
生物活性
The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGFE has been determined to be in the range of 5 - 20 ng/ml. Specific activity: 2 x 105 units/mg.
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纯度
> 90 % SDS-PAGE.
Purity: > 90%, by SDS-PAGE and visualised by silver stain. Endotoxin level: < 0.1 ng per ug of VEGFE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHHSSGLVPRGSHDSTKTWSEVFENSGCKPRPMVFRVHDEHPE LTSQRFNPPCVTLMRCGGCCNDESLECVPTEEANVTMQLMGASVSGGNGM QHLSFVEHKKCDCKPPLTTTPPTTTRPPRRRR -
氨基酸
21 to 81 -
标签
His tag N-Terminus -
额外的序列信息
Recombinant VEGFE with an N-terminal His-tag sequence and a thrombin cleavage site. GenBank accession No. AF106020.
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技术指标
Our Abpromise guarantee covers the use of ab53679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
Like VEGFA, VEGFE was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGFA, VEGFE can not bind to VEGF receptor-1 (Flt-1). Furthermore VEGFE can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGFE is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶The lyophilised VEGFE is soluble in water and most aqueous buffers. The lyophilised VEGFE should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.
常规信息
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相关性
Based on sequence similarity to VEGFA, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGFA and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGFE proteins (Dehio et al., 1999; Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGFE is distinct from the previously described VEGFs but most closely related to VEGFA.
图片
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SDS-PAGE - Recombinant human VEGFE protein (ab53679)SDS-PAGE analysis of recombinant ov-VEGF-E. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.
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Functional Studies - Recombinant human VEGFE protein (ab53679)Stimulation of cell proliferation in primary human umbilical vein endothelial cells (HUVEC) by recombinant ov-VEGF-E and ov-HB-VEGF-E. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab53679 尚未被引用在任何文献中。
