JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB55566

Recombinant Human VEGFA protein

Recombinant Human VEGFA protein

5

(1 Review)

|

(6 Publications)

Recombinant Human VEGFA protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

查看别名

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

1 Images
SDS-PAGE - Recombinant Human VEGFA protein (AB55566)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human VEGFA protein (AB55566)

ab55566 on SDS-PAGE

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

WB, SDS-PAGE

applications

生物活性

No

访问

P15692

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.242% Tris

storage-buffer

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab55566 can be used as a WB positive control in conjunction with <a href='/products/unavailable/vegfa-antibody-mabcam-68334-ab68334'>ab68334</a>.</p>" } } }
style
left-column

您可能对以下产品感兴趣:

AB222510

Human VEGF ELISA Kit

5

1 Reviews

查看产品
为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

style
left-column
style
left-column

序列信息

[{"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":"23.6 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P15692","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
style
left-column

特殊说明

形式

Liquid

附加说明

Proprietary purification method

style
left-column

常规信息

功能

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

序列相似性

Belongs to the PDGF/VEGF growth factor family.

翻译后修饰

Vascular endothelial growth factor A, long form. Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.

style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
See full target information VEGFA
style
left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 13: PubMed38004368

2023

Mathematical Modeling of Non-Small-Cell Lung Cancer Biology through the Experimental Data on Cell Composition and Growth of Patient-Derived Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Rushan Sulimanov,Konstantin Koshelev,Vladimir Makarov,Alexandre Mezentsev,Mikhail Durymanov,Lilian Ismail,Komal Zahid,Yegor Rumyantsev,Ilya Laskov

Cell death & disease 11:1016 PubMed33247124

2020

Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Aisong Guo,Yuanyuan Tu,Wendie Li,Lele Li,Wangrui Liu,Yuanyuan Ju,Yamei Zhou,Aimin Sang,Manhui Zhu

Journal of medicinal food : PubMed32614624

2020

Angiogenesis is Differentially Modulated by Anthocyanin and Phenolic Acid Extracts from Wild Blueberry () Through PI3K Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Panagiotis Tsakiroglou,James Weber,Sharon Ashworth,Cristian Del Bo',Dorothy Klimis-Zacas

Cell death & disease 9:906 PubMed30185783

2018

Polyphyllin I suppresses the formation of vasculogenic mimicry via Twist1/VE-cadherin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xiao,Weilong Zhong,Jianmin Zhao,Baoxin Qian,Huijuan Liu,Shuang Chen,Kailiang Qiao,Yueyang Lei,Shumin Zong,Hongzhi Wang,Yuan Liang,Heng Zhang,Jing Meng,Honggang Zhou,Tao Sun,Yanrong Liu,Cheng Yang

PloS one 10:e0144823 PubMed26657744

2015

Adipose-Derived Stromal Vascular Fraction Cell Effects on a Rodent Model of Thin Endometrium.

Applications

WB

Species

Unspecified reactive species

Robert K Hunter,Chris D Nevitt,Jeremy T Gaskins,Bradley B Keller,Henry C L Bohler,Amanda J LeBlanc

Cardiovascular research 93:111-9 PubMed22002459

2011

ARNO regulates VEGF-dependent tissue responses by stabilizing endothelial VEGFR-2 surface expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna K Mannell,Joachim Pircher,Daniel I Chaudhry,Stefan K C Alig,Elisabeth G Koch,Ramona Mettler,Ulrich Pohl,Florian Krötz
View all publications
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
style
right-column

关联产品

Select an associated product type
Alternative Product
Proteins & Peptides

AB50078

Recombinant human VEGFA protein

proteins-peptides

recombinant-human-vegfa-protein-ab50078

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB217394

Recombinant human VEGFA protein (Animal Free)

proteins-peptides

recombinant-human-vegfa-protein-animal-free-ab217394

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB187239

Recombinant human VEGFA protein (Animal Free)

proteins-peptides

recombinant-human-vegfa-protein-animal-free-ab187239

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB155722

Recombinant human VEGFA protein (Active)

proteins-peptides

recombinant-human-vegfa-protein-active-ab155722

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB155740

Recombinant human VEGFA protein (Active)

proteins-peptides

recombinant-human-vegfa-protein-active-ab155740

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB220553

Recombinant human VEGFA protein (Active)

proteins-peptides

recombinant-human-vegfa-protein-active-ab220553

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB191623

Recombinant mouse VEGFA protein (Active)

proteins-peptides

recombinant-mouse-vegfa-protein-active-ab191623

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB53824

Recombinant human VEGFA protein

proteins-peptides

recombinant-human-vegfa-protein-ab53824

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB224845

Recombinant Pig VEGFA protein (His tag)

proteins-peptides

recombinant-pig-vegfa-protein-his-tag-ab224845

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB46160

VEGFA peptide

proteins-peptides

vegfa-peptide-ab46160

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB185265

Recombinant mouse VEGFA protein (Active)

proteins-peptides

recombinant-mouse-vegfa-protein-active-ab185265

0

(0 reviews)

Alternative Product
Proteins & Peptides

AB92846

Recombinant rat VEGFA protein

proteins-peptides

recombinant-rat-vegfa-protein-ab92846

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com