重组人VEGF Receptor 1蛋白(ab54346)
Key features and details
- Expression system: Insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人VEGF Receptor 1蛋白
参阅全部 VEGF Receptor 1 蛋白酶 -
生物活性
The activity of sVEGFR-1 was determined by its ability to inhibit the VEGF-A-induced proliferation of HUVECs.
Endotoxin Level: < 0.1 ng per µg of sFlt-1
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纯度
> 90 % SDS-PAGE.
Purified by affinity chromatography. -
表达系统
Insect cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
SGSKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERL SITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAI YIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPL DTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHR QTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNK RASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNT SVHIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGL PATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIV NVKPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHN HSEARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVV ADSRISGIYICIASNKVGTVGRNISFYITDVPNGFHVNLEKMPTEGEDLK LSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIM NVSLQDSGTYACRARNVYTGEEILQKKEITIRGEHCNKKAVFSRISKFKS TRNDCTTQSNVKH -
氨基酸
25 to 688 -
额外的序列信息
VEGF Receptor 1 isoform 2 without the signal peptide
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相关产品
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Positive Controls
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Recombinant Protein
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab54346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
The lyophilized sVEGFR-1 is soluble in water and most aqueous buffers
Molecular weight: 96 kDa
Lyophilized from 50mM acetic acid
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶This protein should be reconstituted in PBS to a concentration not lower than 100 ng/ml.
常规信息
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别名
- EC 2.7.10.1
- FLT
- FLT 1
see all -
功能
Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis. -
组织特异性
Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
图片
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Functional Studies - Recombinant human VEGF Receptor 1 protein (ab54346)
FLT-1 Sandwich-ELISA using recombinant human soluble FLT-1 as standard. Mouse anti-human FLT-1 was used as capture antibody, Biotinylated rabbit anti-human FLT-1 was used for detection.
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SDS-PAGE - Recombinant human VEGF Receptor 1 protein (ab54346)SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie stain. -
Functional Studies - Recombinant human VEGF Receptor 1 protein (ab54346)Inhibition of the VEGF165-induced proliferation in HUVECs by soluble VEGFR-1/Flt-1. VEGF165 (10ng/ml) was preincubated with increasing amounts of sVEGFR-1/Flt-1 for 1h and then added to the cells.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab54346 被引用在 1 文献中.
- Gao X et al. A comparison of BMP2 delivery by coacervate and gene therapy for promoting human muscle-derived stem cell-mediated articular cartilage repair. Stem Cell Res Ther 10:346 (2019). PubMed: 31771623
