重组人VEGF 121B蛋白(ab61872)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人VEGF 121B蛋白
参阅全部 VEGF 121B 蛋白酶 -
生物活性
Biological Activity: The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4 ng/ml.
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纯度
> 95 % SDS-PAGE.
Purity greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab61872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
Aliquot and freeze at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶It is recommended to reconstitute the lyophilized VEGF in sterile H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
常规信息
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别名
- MVCD1
- Vascular endothelial growth factor A
- Vascular permeability factor
see all -
相关性
VEGFA is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF 121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates. -
细胞定位
Secreted
图片
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Functional Studies - Recombinant human VEGF 121B protein (ab61872)Functional analysis of ab61872
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SDS-PAGE - Recombinant human VEGF 121B protein (ab61872)SDS PAGE analysis of ab61872 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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Functional Studies - Recombinant human VEGF 121B protein (ab61872)ab61872 used in Functional Studies.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab61872 尚未被引用在任何文献中。
