AB219443

重组人VCAM1蛋白(His tag)

Recombinant human VCAM1 protein (His tag)

Bioactive
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Recombinant human VCAM1 protein (His tag) is a Human Fragment protein, in the 25 to 698 aa range, expressed in Baculovirus infected insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

查看别名

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

1 Images

SDS-PAGE - Recombinant human VCAM1 protein (His tag) (AB219443)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human VCAM1 protein (His tag) (AB219443)

15% SDS-PAGE analysis of ab219443 (3μg).

关键信息

纯度

>90% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

Baculovirus infected insect cells

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Measured by the ability of the immobilized protein to support the adhesion of U937 Human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates (VCAM1 at 10ug/ml), more than 70% of the cells are adhered.

访问

P19320

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"ADPEFFKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLIST","proteinLength":"Fragment","predictedMolecularWeight":"75.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":698,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"P19320","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

True

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.

Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

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特殊说明

形式

Liquid

附加说明

Affinity purified

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常规信息

功能

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).

翻译后修饰

Cleaved by the metalloproteinase ADAM17 to generate the soluble form.. Sialoglycoprotein.. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination; leading to proteasomal degradation.

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information VCAM1

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重组人VCAM1蛋白(His tag)

Recombinant human VCAM1 protein (His tag)

SDS-PAGE - Recombinant human VCAM1 protein (His tag) (AB219443)

关键信息

纯度

内毒素水平

表达系统

标签

应用

生物活性

生物学活性

访问

不含动物源

不含载体蛋白

种属

存储溶液