AB101214

重组人 VAPB 蛋白 (His tag N-Terminus)

Name: 重组人VAPB蛋白 (ab101214)| Abcam中文官网
Brand: Abcam Limited
SKU: AB101214
Price: 3852 CNY
Availability: InStock

Recombinant Human VAPB protein (His tag N-Terminus)

Be the first to review this product! Submit a review

(0 Publication)

Recombinant Human VAPB protein (His tag N-Terminus) is a Human Fragment protein, in the 1 to 222 aa range, expressed in Escherichia coli, with >90%, suitable for Mass Spec, SDS-PAGE.

查看别名

UNQ484/PRO983, VAPB, Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C

1 Images

SDS-PAGE - Recombinant Human VAPB protein (His tag N-Terminus) (AB101214)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human VAPB protein (His tag N-Terminus) (AB101214)

ab101214 at 3 µg analysed by 15% SDS PAGE.

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

应用

SDS-PAGE, Mass Spec

applications

生物活性

访问

O95292

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Molecular size on SDS-PAGE will appear higher.</p>" } } }

style

left-column

style

left-column

序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFAPTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDGLRMRKTVQSNSPISALAPTGKEEGLST","proteinLength":"Fragment","predictedMolecularWeight":"27.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":222,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95292","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

style

left-column

性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

VAMP-associated protein B (VAPB) also known as VAP-B is a protein with a mass of approximately 27 kDa. It plays a role in membrane trafficking and lipid transport. VAPB accomplishes this by interacting with various vesicle-associated membrane protein (VAMP) family members. It is expressed in multiple tissues including the brain liver and muscle. VAPB localizes to the endoplasmic reticulum where it facilitates its mechanical functions.

Biological function summary

The protein assists in maintaining cellular homeostasis by influencing lipid transport and membrane composition. VAPB forms part of a larger protein complex that includes other lipid-binding proteins and is essential for the regulation of lipid-dependent signaling pathways. This complex allows VAPB to perform its biological functions effectively within cells.

Pathways

VAPB participates in critical pathways like the unfolded protein response (UPR) and ER-associated degradation (ERAD). These pathways ensure proper protein folding and degradation. VAPB interacts with other important proteins such as VAMP family members to convey signals and manage lipid distribution. Disruptions in these pathways can affect cellular functions evidencing VAPB's integral role.

VAPB has strong connections to certain neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Mutations in the VAPB gene have been linked to the familial form of ALS impacting the protein's function and leading to disease progression. VAPB associates with proteins like TDP-43 which also play a role in neurodegenerative disease mechanisms establishing its critical involvement in these conditions.

style

left-column

特殊说明

形式

Liquid

附加说明

ab101214 was purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

style

left-column

常规信息

功能

Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMed : 32344433, PubMed : 33124732). Interacts with STARD3 in a FFAT motif phosphorylation dependent manner (PubMed : 33124732). Via interaction with WDR44 participates in neosynthesized protein export (PubMed : 32344433). Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity (PubMed : 16891305, PubMed : 20940299). Involved in cellular calcium homeostasis regulation (PubMed : 22131369).

序列相似性

Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.

style

left-column

产品实验方案

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

See full target information VAPB

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组人 VAPB 蛋白 (His tag N-Terminus)

Recombinant Human VAPB protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human VAPB protein (His tag N-Terminus) (AB101214)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液