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AB127357

重组人USP33蛋白

Recombinant Human USP33 protein

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Recombinant Human USP33 protein is a Human Fragment protein, in the 71 to 303 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

查看别名

KIAA1097, VDU1, USP33, Ubiquitin carboxyl-terminal hydrolase 33, Deubiquitinating enzyme 33, Ubiquitin thioesterase 33, Ubiquitin-specific-processing protease 33, VHL-interacting deubiquitinating enzyme 1, hVDU1

关键信息

纯度

>85% SDS-PAGE

表达系统

Escherichia coli

标签

His-DHFR tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q8TEY7

不含动物源

No

不含载体蛋白

No

种属

Human

复溶

Reconstitute in water

存储溶液

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"26.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":303,"aminoAcidStart":71,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q8TEY7","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

USP33 also known as ubiquitin-specific peptidase 33 is an important enzyme involved in the ubiquitin-proteasome system. It has a molecular mass of about 105 kDa. USP33 acts as a deubiquitinating enzyme meaning it removes ubiquitin from target proteins which stabilizes or changes their function. You find USP33 expressed in various tissues with significant levels in the brain lung and heart. Alternate names include VDU1 and a few other names reflecting variations in biological contexts.
Biological function summary

USP33 plays roles in cellular processes like signal transduction and intracellular trafficking often as part of protein complexes. Its enzymatic activity influences the regulation of proteins tasked with moving cellular components cytoskeletal dynamics and response to hypoxia. Acting on proteins such as beta-arrestin2 USP33 influences the endocytosis and recycling of G-protein-coupled receptors (GPCRs). This role allows it to impact multiple receptor-mediated signaling pathways.

Pathways

USP33 participates in critical cellular pathways including the hypoxia response and Wnt signaling. It interacts with hypoxia-inducible factor 1-alpha (HIF-1α) modifying its stability and affecting cellular response to low oxygen levels. In the Wnt signaling pathway it modulates the activity of beta-catenin through deubiquitination which is essential for cell proliferation and differentiation. These roles highlight USP33's position within wide networks of cellular regulation influencing different proteomic interactions and outcomes.

USP33 has been linked to certain cancers and neurodegenerative diseases. Its interaction with HIF-1α connects it to tumor progression as altered USP33 expression can influence cancer cell survival in hypoxic conditions. In neurodegenerative diseases like Parkinson's changes in the deubiquitination activity of USP33 may lead to protein aggregation. Its interactions with proteins such as alpha-synuclein are relevant as these proteins play a significant role in disease pathology. Understanding USP33's functions and associations offers insight into potential therapeutic targets.
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特殊说明

形式

Lyophilized

附加说明

Purified via His tag

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常规信息

功能

Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

序列相似性

Belongs to the peptidase C19 family. USP20/USP33 subfamily.

翻译后修饰

Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.

亚细胞定位

Cytoskeleton

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产品实验方案

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靶点信息

Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
See full target information USP33
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搭配使用产品

Primary Antibodies

AB237510

Anti-USP33 antibody

Immunocytochemistry/ Immunofluorescence - Anti-USP33 antibody (AB237510)

  • 0
  • 0
Primary Antibodies

AB197246

Anti-DEPDC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEPDC1 antibody (AB197246)

  • 0
  • 0
Cell Lines & Lysates

AB266103

Human USP33 knockout HEK-293T cell line

Sanger Sequencing - Human USP33 knockout HEK-293T cell line (AB266103)

  • 0
  • 0
Cell Lines & Lysates

AB263411

Human USP33 knockout HEK-293T cell lysate

Sanger Sequencing - Human USP33 knockout HEK-293T cell lysate (AB263411)

  • 0
  • 0

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