重组人USP20蛋白(Tagged) (ab271783)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人USP20蛋白(Tagged)
参阅全部 USP20 蛋白酶 -
生物活性
Specific Activity: 10 pmol/min/μg
Assay Conditions: 50mM Tris-HCl, 0.5mM EDTA, 0.05% Tween 20, 1mM DTT, 500nM Ubiquitin-AMC. Incubate at room temperature for 30 minutes. Fluorescence intensity is measured at exc/em of 360nm/460nm using Tecan M1000 plate reader.
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纯度
>= 90 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
GDSRDLCPHL DSIGEVTKED LLLKSKGTCQ SCGVTGPNLW ACLQVACPYV GCGESFADHS TIHAQAKKHN LTVNLTTFRL WCYACEKEVF LEQRLAAPLL GSSSKFSEQD SPPPSHPLKA VPIAVADEGE SESEDDDLKP RGLTGMKNLG NSCYMNAALQ ALSNCPPLTQ FFLECGGLVR TDKKPALCKS YQKLVSEVWH KKRPSYVVPT SLSHGIKLVN PMFRGYAQQD TQEFLRCLMD QLHEELKEPV VATVALTEAR DSDSSDTDEK REGDRSPSED EFLSCDSSSD RGEGDGQGRG GGSSQAETEL LIPDEAGRAI SEKERMKDRK FSWGQQRTNS EQVDEDADVD TAMAALDDQP AEAQPPSPRS SSPCRTPEPD NDAHLRSSSR PCSPVHHHEG HAKLSSSPPR ASPVRMAPSY VLKKAQVLSA GSRRRKEQRY RSVISDIFDG SILSLVQCLT CDRVSTTVET FQDLSLPIPG KEDLAKLHSA IYQNVPAKPG ACGDSYAAQG WLAFIVEYIR RFVVSCTPSW FWGPVVTLED CLAAFFAADE LKGDNMYSCE RCKKLRNGVK YCKVLRLPEI LCIHLKRFRH EVMYSFKINS HVSFPLEGLD LRPFLAKECT SQITTYDLLS VICHHGTAGS GHYIAYCQNV INGQWYEFDD QYVTEVHETV VQNAEGYVLF YRKSSEEAMR ERQQVVSLAA MREPSLLRFY VSREWLNKFN TFAEPGPITN QTFLCSHGGI PPHKYHYIDD LVVILPQNVW EHLYNRFGGG PAVNHLYVCS ICQVEIEALA KRRRIEIDTF IKLNKAFQAE ESPGVIYCIS MQWFREWEAF VKGKDNEPPG PIDNSRIAQV KGSGHVQLKQ GADYGQISEE TWTYLNSLYG GGPEIAIRQS VAQPLGPENL HGEQKIEAET RAV -
预测分子量
104 kDa including tags -
氨基酸
2 to 914 -
标签
His tag N-Terminus , DDDDK tag C-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab271783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
100ug/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Deubiquitinating enzyme 20
- hVDU2
- KIAA1003
see all -
功能
Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
序列相似性
Belongs to the peptidase C19 family. USP20/USP33 subfamily.
Contains 2 DUSP domains.
Contains 1 UBP-type zinc finger. -
结构域
The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue. -
翻译后修饰
Ubiquitinated via a VHL-dependent pathway for proteasomal degradation. -
细胞定位
Cytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human USP20 protein (Tagged) (ab271783)
SDS-PAGE analysis of ab271783.
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Functional Studies - Recombinant Human USP20 protein (Tagged) (ab271783)Functional studies analysis of ab271783 was 10 pmol/min/μg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271783 尚未被引用在任何文献中。