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AB139775

重组人 USP14/TGT 蛋白 (His tag N-Terminus)

Recombinant Human USP14/TGT protein (His tag N-Terminus)

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Recombinant Human USP14/TGT protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 494 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

TGT, USP14, Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14

1 Images
SDS-PAGE - Recombinant Human USP14/TGT protein (His tag N-Terminus) (AB139775)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human USP14/TGT protein (His tag N-Terminus) (AB139775)

15% SDS-PAGE analysis of ab139775 (3μg).

关键信息

纯度

>90% SDS-PAGE

ab139775 is purified using conventional chromatography techniques.

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

P54578

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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搭配使用产品

Primary Antibodies

AB192618

Anti-USP14/TGT antibody [EPR15943] - C-terminal

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-USP14/TGT antibody [EPR15943] - C-terminal (AB192618)

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Proteins & Peptides

AB196393

Recombinant human PADI4 / PAD4 protein (DDDDK tag C-Terminus)

Functional Studies - Recombinant human PADI4 / PAD4 protein (DDDDK tag C-Terminus) (AB196393)

  • 0
  • 0
Proteins & Peptides

AB167915

Recombinant Human GIMAP6 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GIMAP6 protein (His tag N-Terminus) (AB167915)

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  • 0
Proteins & Peptides

AB268701

Recombinant Mouse KDM4B / JMJD2B protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Mouse KDM4B / JMJD2B protein (Tagged) (AB268701)

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

This product was previously labelled as USP14
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序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ","proteinLength":"Full Length","predictedMolecularWeight":"58.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":494,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P54578","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

USP14 also known as Ubiquitin Specific Peptidase 14 is a deubiquitinating enzyme that plays a mechanical role in the ubiquitin-proteasome system. This enzyme has a molecular mass around 56 kDa and is essential for proteasome-mediated degradation activities. It is broadly expressed across many tissues but shows significant presence in the nervous system. Often people refer to USP14 as TGT in the context of certain studies or databases.
Biological function summary

USP14 functions in regulating protein turnover by removing ubiquitin from substrate proteins impacting their stability and degradation. USP14 is part of a larger protein complex associated with the proteasome specifically binding to the 19S regulatory particle. It is important in maintaining cellular protein homeostasis by editing ubiquitin chains on target proteins prior to degradation.

Pathways

USP14 participates in ubiquitin-proteasome pathways and ER-associated degradation pathway. In these processes it works closely with other proteasome proteins such as Rpn11 which also removes ubiquitin from proteins. The regulation of these pathways by USP14 directly affects protein quality control in cells influencing processes like cell cycle and stress response.

USP14 has associations with neurodegenerative diseases such as Alzheimer's and Parkinson’s. It affects disease progression by modulating levels of proteins that aggregate in these conditions. Connections between USP14 and proteins such as tau and α-synuclein highlight its contribution to the pathological features seen in these disorders. Further research into USP14's role may help to uncover new therapeutic strategies for these diseases.
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常规信息

功能

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed : 35145029). Ensures the regeneration of ubiquitin at the proteasome (PubMed : 18162577, PubMed : 28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed : 18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed : 19106094). Also serves as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed : 19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed : 27666593). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (PubMed : 35145029).

序列相似性

Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

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产品实验方案

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靶点信息

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed : 35145029). Ensures the regeneration of ubiquitin at the proteasome (PubMed : 18162577, PubMed : 28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed : 18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed : 19106094). Also serves as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed : 19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed : 27666593). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (PubMed : 35145029).
See full target information USP14
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