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AB160279

重组人 USP13 蛋白 (GST tag N-Terminus)

Recombinant Human USP13 protein (GST tag N-Terminus)

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Recombinant Human USP13 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 863 aa range, expressed in Wheat germ, suitable for ELISA, WB.

查看别名

ISOT3, USP13, Ubiquitin carboxyl-terminal hydrolase 13, Deubiquitinating enzyme 13, Isopeptidase T-3, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, ISOT-3

1 Images
SDS-PAGE - Recombinant Human USP13 protein (GST tag N-Terminus) (AB160279)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human USP13 protein (GST tag N-Terminus) (AB160279)

ab160279 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

WB, ELISA

applications

生物活性

No

访问

Q92995

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MQRRGALFGMPGGSGGRKMAAGDIGELLVPHMPTIRVPRSGDRVYKNECAFSYDSPNSEGGLYVCMNTFLAFGREHVERHFRKTGQSVYMHLKRHVREKVRGASGGALPKRRNSKIFLDLDTDDDLNSDDYEYEDEAKLVIFPDHYEIALPNIEELPALVTIACDAVLSSKSPYRKQDPDTWENELPVSKYANNLTQLDNGVRIPPSGWKCARCDLRENLWLNLTDGSVLCGKWFFDSSGGNGHALEHYRDMGYPLAVKLGTITPDGADVYSFQEEEPVLDPHLAKHLAHFGIDMLHMHGPENGLQDNDIKLRVSEWEVIQESGTKLKPMYGPGYTGLKNLGNSCYLSSVMQAIFSIPEFQRAYVGNLPRIFDYSPLDPTQDFNTQMTKLGHGLLSGQYSKPPVKSELIEQVMKEEHKPQQNGISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSENPSDVFRFLVEERIQCCQTRKVRYAERVDYLMQLPVAMEAATNKDELIAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDGFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDCLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":863,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q92995","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

USP13 also known as Ubiquitin Specific Protease 13 functions as a deubiquitinating enzyme. It is important in removing ubiquitin molecules from protein substrates which prevents them from being degraded by the proteasome system. USP13 has a molecular mass of approximately 97 kDa and shows expression in several tissues including brain heart and kidney. This indicates its diverse role in human physiology.
Biological function summary

USP13 participates in maintaining protein homeostasis and modulates protein stability. It forms complexes with specific substrate proteins to execute its function efficiently. Through deubiquitinating actions it regulates the levels of proteins involved in cellular signaling apoptosis and cell cycle control. The enzyme influences processes that are important for cell survival and adaptation to stress.

Pathways

USP13 plays significant roles within the ubiquitin-proteasome system and the mitophagy pathway. It cooperates with proteins like Parkin in mitochondrial quality control by stabilizing target proteins necessary for mitophagy. Through interaction with key players in these pathways USP13 ensures the maintenance of cellular health and proper degradation of damaged or misfolded proteins.

USP13 has associations with cancer and neurodegenerative diseases. Overexpression of USP13 might contribute to tumor progression by inhibiting degradation of oncoproteins. It also shows connections to disorders like Parkinson's disease where it interacts with proteins such as Parkin and PINK1 which are important for mitochondrial function. These interactions highlight USP13's potential as a target for therapeutic interventions.
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特殊说明

形式

Liquid

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常规信息

功能

Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy, endoplasmic reticulum-associated degradation (ERAD), cell cycle progression or DNA damage response (PubMed : 21571647, PubMed : 32772043, PubMed : 33592542). Component of a regulatory loop that controls autophagy and p53/TP53 levels : mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Alternatively, forms with NEDD4 a deubiquitination complex, which subsequently stabilizes VPS34 to promote autophagy (PubMed : 32101753). Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity : binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Regulates the cell cycle progression by stabilizing cell cycle proteins such as SKP2 and AURKB (PubMed : 32772043). In addition, plays an important role in maintaining genomic stability and in DNA replication checkpoint activation via regulation of RAP80 and TOPBP1 (PubMed : 33592542). Deubiquitinates the multifunctional protein HMGB1 and subsequently drives its nucleocytoplasmic localization and its secretion (PubMed : 36585612). Positively regulates type I and type II interferon signalings by deubiquitinating STAT1 but negatively regulates antiviral response by deubiquitinating STING1 (PubMed : 23940278, PubMed : 28534493).

序列相似性

Belongs to the peptidase C19 family.

翻译后修饰

Phosphorylated by AURKB at Ser-114; leading to stabilization of cell cycle proteins such as SKP2 and AURKB, but not MCL1.

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产品实验方案

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靶点信息

Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy, endoplasmic reticulum-associated degradation (ERAD), cell cycle progression or DNA damage response (PubMed : 21571647, PubMed : 32772043, PubMed : 33592542). Component of a regulatory loop that controls autophagy and p53/TP53 levels : mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Alternatively, forms with NEDD4 a deubiquitination complex, which subsequently stabilizes VPS34 to promote autophagy (PubMed : 32101753). Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity : binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Regulates the cell cycle progression by stabilizing cell cycle proteins such as SKP2 and AURKB (PubMed : 32772043). In addition, plays an important role in maintaining genomic stability and in DNA replication checkpoint activation via regulation of RAP80 and TOPBP1 (PubMed : 33592542). Deubiquitinates the multifunctional protein HMGB1 and subsequently drives its nucleocytoplasmic localization and its secretion (PubMed : 36585612). Positively regulates type I and type II interferon signalings by deubiquitinating STAT1 but negatively regulates antiviral response by deubiquitinating STING1 (PubMed : 23940278, PubMed : 28534493).
See full target information USP13
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搭配使用产品

Primary Antibodies

AB109264

Anti-USP13 antibody [EPR4348]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-USP13 antibody [EPR4348] (AB109264)

  • 4
  • 2
Proteins & Peptides

AB163562

Recombinant Human USP31 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human USP31 protein (GST tag N-Terminus) (AB163562)

  • 0
  • 0
Proteins & Peptides

AB198669

Recombinant human USP10 protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human USP10 protein (DDDDK tag N-Terminus) (AB198669)

  • 0
  • 0
Proteins & Peptides

AB161390

Recombinant Human UBP43/USP18 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human UBP43/USP18 protein (GST tag N-Terminus) (AB161390)

  • 0
  • 0

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