AB269114

重组人USP11蛋白(Tagged)

Name: 重组人USP11蛋白(Tagged) (ab269114)| Abcam中文官网
Brand: Abcam Limited
SKU: AB269114
Price: 2893 CNY
Availability: InStock

Recombinant human USP11 protein (Tagged)

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(1 Publication)

Recombinant human USP11 protein (Tagged) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab269114 protein is 136 kDa.

- Save time and ensure accurate results - use our human USP11 protein (GST-tag) as a control
- Optimal protein bioactivity and stability

查看别名

UHX1, USP11, Ubiquitin carboxyl-terminal hydrolase 11, Deubiquitinating enzyme 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11

2 Images

Functional Studies - Recombinant human USP11 protein (Tagged) (AB269114)

FuncS

Supplier Data

Functional Studies - Recombinant human USP11 protein (Tagged) (AB269114)

The specific activity of ab269114 was determined to be 47 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.

SDS-PAGE - Recombinant human USP11 protein (Tagged) (AB269114)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human USP11 protein (Tagged) (AB269114)

SDS-PAGE gel of ab269114.

关键信息

纯度

>90% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Specific activity of ab269114 was determined to be 47 nmol/min/mg in a DUB assay using recombinant human ubiquitin-based proluciferin substrate.

访问

P51784

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Ensure the validity of your result using our bioactive recombinant human USP11 protein (GST-Tagged) ab269114 as a control in SDS-PAGE.

Check out our protein gel staining guide for SDS-PAGE here

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序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":"136 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P51784","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Dry Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

True

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

USP11 also known as ubiquitin specific peptidase 11 is a deubiquitinating enzyme with a molecular mass of approximately 110 kDa. It removes ubiquitin molecules from target proteins regulating their stability and function. This enzyme is part of the USP family and exhibits strong expression in tissues like the testes brain and thyroid. The deubiquitination process is significant in maintaining protein homeostasis by recycling ubiquitins and controlling protein degradation.

Biological function summary

Ubiquitin-specific peptidases such as USP11 participate in diverse cellular functions including DNA repair signal transduction and cell cycle progression. USP11 often operates as part of a larger regulatory complex. It modulates DNA repair by interacting with proteins involved in the repair processes such as BRCA2. Through its deubiquitination activity USP11 significantly influences cellular response to DNA damage and helps to maintain genomic stability.

Pathways

USP11 plays a role in key mechanisms like the DNA damage response and NF-kB signaling pathways. It interacts with proteins involved in these pathways offering a layer of regulation. In the DNA damage response pathway USP11 associates with BRCA2 assisting in the repair of double-stranded breaks. In the NF-kB signaling pathway its activity can affect the degradation of IkB which allows the transcription factor NF-kB to enter the nucleus and promote gene expression.

Aberrations in USP11 function have been linked to cancer and neurodegenerative diseases. In cancer alterations in USP11 might contribute to tumorigenesis by affecting the DNA repair pathways especially through the BRCA2 protein interactions. Neurodegenerative diseases may also be connected to USP11 dysfunction due to its role in the degradation of proteins that accumulate in these conditions. The enzyme's interaction with various signaling proteins suggests a potential target for therapeutic interventions in these diseases.

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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed : 12084015, PubMed : 15314155, PubMed : 17897950, PubMed : 19874889, PubMed : 20233726, PubMed : 24724799, PubMed : 28992046). Inhibits the degradation of target proteins by the proteasome (PubMed : 12084015). Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro) (PubMed : 24724799). Plays a role in the regulation of pathways leading to NF-kappa-B activation (PubMed : 17897950, PubMed : 19874889). Plays a role in the regulation of DNA repair after double-stranded DNA breaks (PubMed : 15314155, PubMed : 20233726). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (PubMed : 20601937). Promotes cell proliferation by deubiquitinating phosphorylated E2F1 (PubMed : 28992046).

序列相似性

Belongs to the peptidase C19 family.

亚细胞定位

Nucleus

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information USP11

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer communications (London, England) 42:1008-1027 PubMed35975322

2022

A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Juhong Wang,Fei Shao,Yannan Yang,Wei Wang,Xueying Yang,Renda Li,Hong Cheng,Sijin Sun,Xiaoli Feng,Yibo Gao,Jie He,Zhimin Lu

View all publications

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重组人USP11蛋白(Tagged)

Recombinant human USP11 protein (Tagged)

Functional Studies - Recombinant human USP11 protein (Tagged) (AB269114)

SDS-PAGE - Recombinant human USP11 protein (Tagged) (AB269114)

关键信息

纯度

表达系统

标签

应用

生物活性

生物学活性

访问

不含动物源

不含载体蛋白

种属

存储溶液