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AB132351

重组人 USP1 蛋白 (GST tag N-Terminus)

Recombinant Human USP1 protein (GST tag N-Terminus)

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Recombinant Human USP1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 785 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

查看别名

Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1, Ubiquitin thioesterase 1, Ubiquitin-specific-processing protease 1, hUBP, USP1

1 Images
SDS-PAGE - Recombinant Human USP1 protein (GST tag N-Terminus) (AB132351)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human USP1 protein (GST tag N-Terminus) (AB132351)

12.5% SDS-PAGE analysis of ab132351 stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, SDS-PAGE, WB

applications

生物活性

No

访问

O94782

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL","proteinLength":"Full Length","predictedMolecularWeight":"114.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":785,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O94782","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

USP1 also known as ubiquitin-specific peptidase 1 is a deubiquitinating enzyme with a molecular mass of approximately 88 kDa. It removes ubiquitin from protein substrates especially those involved in DNA repair processes. USP1 is widely expressed in human tissues with high expression in the testis and moderate levels in the lungs brain and heart. It forms a complex with UAF1 which enhances its catalytic activity and stability.
Biological function summary

USP1 plays a critical part in maintaining genomic stability by regulating key proteins in the DNA damage response. It specifically deubiquitinates FANCD2 and PCNA proteins important for the Fanconi anemia pathway and translesion DNA synthesis respectively. This action prevents the accumulation of ubiquitinated proteins ensuring proper progression of DNA repair processes. The interaction with UAF1 forms a functional complex important for its activity in vivo.

Pathways

USP1 is significant in the Fanconi anemia (FA) and translesion synthesis pathways. Its interaction with FANCD2 involves DNA repair by promoting error-free replication across DNA lesions. USP1 also acts on proliferating cell nuclear antigen (PCNA) a protein important for translesion synthesis that enables DNA replication to occur past unrepaired lesions. These pathways highlight USP1’s importance in DNA repair and replication linking it to other proteins like RAD18 which is involved in PCNA ubiquitination.

Research implicates USP1 in cancer and Fanconi anemia. In certain cancers altered USP1 expression can lead to increased DNA repair activity contributing to chemoresistance. USP1’s interaction with FANCD2 makes it pertinent to Fanconi anemia where its malfunction can cause DNA repair defects associated with the disease. Understanding USP1’s role alongside proteins like BRCA1 which interacts with DNA damage repair mechanisms can guide targeted therapeutic strategies.
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特殊说明

形式

Liquid

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常规信息

功能

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed : 15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed : 16531995, PubMed : 20147293). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed : 18082604, PubMed : 26388029).

序列相似性

Belongs to the peptidase C19 family.

翻译后修饰

Autocatalytic cleavage of USP1 following UV irradiation inactivates it, leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.. Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment. Ubiquitinated by the CRL2(KLHDC2) complex following autocatalytic cleavage, leading to its degradation: the CRL2(KLHDC2) complex recognizes the diglycine (Gly-Gly) at the C-terminus.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed : 15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed : 16531995, PubMed : 20147293). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed : 18082604, PubMed : 26388029).
See full target information Ubiquitin carboxyl-terminal hydrolase 1
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搭配使用产品

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Primary Antibodies

AB264221

Anti-USP1 antibody

Immunoprecipitation - Anti-USP1 antibody (AB264221)

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Cell Lines & Lysates

AB263409

Human USP1 knockout HeLa cell lysate

Sanger Sequencing - Human USP1 knockout HeLa cell lysate (AB263409)

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  • 0
Primary Antibodies

AB109451

Anti-PRMT5 antibody [EPR5772]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PRMT5 antibody [EPR5772] (AB109451)

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  • 2
Primary Antibodies

AB154170

Anti-USP5 antibody [EPR10454]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-USP5 antibody [EPR10454] (AB154170)

  • 0
  • 0

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