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AB101548

重组人ULK3蛋白

Recombinant human ULK3 protein

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Recombinant human ULK3 protein is a Human Full Length protein, expressed in Baculovirus infected insect cells, with >95%, suitable for SDS-PAGE, FuncS.

查看别名

Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, ULK3

4 Images
Functional Studies - Recombinant human ULK3 protein (AB101548)
  • FuncS

Unknown

Functional Studies - Recombinant human ULK3 protein (AB101548)

The specific activity of ULK3 (ab101548) was determined to be 210 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human ULK3 protein (AB101548)
  • FuncS

Unknown

Functional Studies - Recombinant human ULK3 protein (AB101548)

Kinase Assay demonstrating specific activity of ab101548.

SDS-PAGE - Recombinant human ULK3 protein (AB101548)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human ULK3 protein (AB101548)

SDS PAGE analysis of ab101548

SDS-PAGE - Recombinant human ULK3 protein (AB101548)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human ULK3 protein (AB101548)

SDS-PAGE showing ab101548 at approximately 51kDa.

关键信息

纯度

>95% SDS-PAGE

表达系统

Baculovirus infected insect cells

标签

His tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

The Specific activity of ab101548 was determined to be 230 nmol/min/mg.

访问

Q6PHR2

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"51 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q6PHR2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ULK3 also known as Unc-51 Like Autophagy Activating Kinase 3 is a serine/threonine-protein kinase with a molecular mass of approximately 58 kDa. This protein plays an important role in mediating cellular processes through phosphorylation. ULK3 shows expression in various tissues including the brain and kidney. Its catalytic activity influences multiple cellular events especially those related to autophagy the cell's degradation pathway for removing damaged organelles and recycling cellular components.
Biological function summary

This protein contributes significantly through its involvement in autophagy regulation. ULK3 acts as a kinase within a multiprotein complex which includes proteins such as ATG13 and FIP200. This complex is essential for initiating autophagy by regulating the autophagosome formation. Its activity can promote cellular survival under nutrient-deprived conditions showcasing its role as an important regulatory component in cellular maintenance and stress responses.

Pathways

ULK3 takes part in critical signaling pathways such as the mTOR signaling pathway and the Hedgehog signaling pathway. In the mTOR pathway ULK3 through its kinase activity acts upstream to regulate the activity of the autophagy machinery. It shares a functional relationship with proteins like mTOR and AMPK. In the Hedgehog pathway ULK3 regulates pathway components impacting cellular growth and differentiation processes. This positions ULK3 as a modulator connecting different signaling pathways to maintain cellular homeostasis.

Researchers have reported associations with cancer and neurodegenerative diseases. Abnormal ULK3 activity can influence tumor growth and progression particularly in its regulatory role in cell proliferation and survival. Studies also suggest an involvement in neurodegenerative diseases where dysregulation in autophagy processes in which ULK3 plays a part contributes to neuronal cell death. In these contexts ULK3's interactions with proteins like mTOR and Beclin-1 may exacerbate disease progression by altering normal cellular processes.
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特殊说明

形式

Liquid

附加说明

The purity was determined to be >95% by densitometry. Affinity purified.

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常规信息

功能

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand : interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH : dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy : following cellular senescence, able to induce autophagy.

序列相似性

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

翻译后修饰

Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU.

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产品实验方案

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靶点信息

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand : interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH : dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy : following cellular senescence, able to induce autophagy.
See full target information Serine/threonine-protein kinase ULK3
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搭配使用产品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB124947

Anti-ULK3 antibody [EPR4888]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ULK3 antibody [EPR4888] (AB124947)

  • 0
  • 0
Proteins & Peptides

AB125656

Recombinant human ULK1 protein

Functional Studies - Recombinant human ULK1 protein (AB125656)

  • 0
  • 0
Assay Kits

AB138879

Universal Kinase Assay Kit (Fluorometric)

Functional Studies - Universal Kinase Assay Kit (Fluorometric) (AB138879)

  • 4
  • 1

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