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AB198417

重组人UBE3A蛋白

Recombinant human UBE3A protein

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Recombinant human UBE3A protein is a Human Full Length protein, in the 2 to 852 aa range, expressed in Baculovirus infected Sf9, with >=81% purity and suitable for Functional studies and SDS-PAGE. The predicted molecular weight of ab198417 protein is 99.8 kDa.

- Save time and ensure accurate results - use our recombinant UBE3A/E6AP protein as a control
- Optimal protein bioactivity, stability and reproducibility

查看别名

E6AP, EPVE6AP, HPVE6A, UBE3A, Ubiquitin-protein ligase E3A, E6AP ubiquitin-protein ligase, HECT-type ubiquitin transferase E3A, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54

2 Images
Functional Studies - Recombinant human UBE3A protein (AB198417)
  • FuncS

Supplier Data

Functional Studies - Recombinant human UBE3A protein (AB198417)

Activity assay using ab198417.

SDS-PAGE - Recombinant human UBE3A protein (AB198417)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human UBE3A protein (AB198417)

4-20% SDS-PAGE analysis of 3 μg ab198417 with Coomassie staining.

关键信息

纯度

>81% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

His-DDDDK tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Assay Conditions: 50 μl of reaction mixture 4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 μg), E2 (2.8 μg), Biotinylated Ubiquitin (1 μg) in 50 mM Tris pH 7.4 was incubated for 30 min at 37°C. Transfer the reaction mixture to a streptavidin-coated plate and incubate for 30 min at 37°C. Add antibody against UBE3A, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

访问

Q05086

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Ensure the validity of your result using our recombinant human UBE3A/E6AP protein ab198417 as a positive control in SDS-PAGE.

The human UBE3A/E6AP protein ab198417 is useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling assays.


Check out our protein gel staining guide for SDS-PAGE here
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序列信息

[{"sequence":"KRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARIDFKDVTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVKQHTKEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGDNNLQKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML","proteinLength":"Full Length","predictedMolecularWeight":"99.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":852,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q05086","tags":[{"tag":"His-DDDDK","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

UBE3A also known as ubiquitin protein ligase E3A is an enzyme with a molecular mass of approximately 100 kDa. It plays a central role in the ubiquitination process by adding ubiquitin to specific substrate proteins. UBE3A is found in many tissues but has high expression levels in the brain especially in neurons. The proper function of UBE3A requires its interaction with the ubiquitin-conjugating enzyme E2 marking target proteins for proteasomal degradation or other cellular processes.
Biological function summary

The role of UBE3A is connected to protein homeostasis and turnover. It is part of the ubiquitin-proteasome system which regulates the degradation of damaged or unneeded proteins. UBE3A does not work alone; it functions as part of a larger E3 ligase complex. This complex selectively identifies and modifies proteins determining their fate within the cell. The precision of UBE3A in these processes is necessary for normal neural development and synaptic plasticity.

Pathways

UBE3A is involved in the synaptic transmission and neuronal signaling pathways. It interacts with proteins such as HERC2 and RAD18 which are involved in DNA damage response and repair. UBE3A's involvement in these pathways highlights its importance in maintaining neuronal health. This protein's activity influences downstream signaling events affecting processes like synaptic function and neuronal communication.

Disruptions in UBE3A activity link to Angelman syndrome and autism spectrum disorders. Angelman syndrome results from the loss of function of the maternal UBE3A gene leading to severe neurological impairments. UBE3A's relationship with proteins like MECP2 involved in gene regulation suggests a broader role in neurodevelopmental disorders. Research continues to explore how UBE3A and its interaction partners contribute to the underlying mechanisms of these diseases.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates (PubMed : 10373495, PubMed : 16772533, PubMed : 19204938, PubMed : 19233847, PubMed : 19325566, PubMed : 19591933, PubMed : 22645313, PubMed : 24273172, PubMed : 24728990, PubMed : 30020076). Several substrates have been identified including the BMAL1, ARC, LAMTOR1, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B (PubMed : 10373495, PubMed : 19204938, PubMed : 19325566, PubMed : 19591933, PubMed : 22645313, PubMed : 24728990, PubMed : 30020076). Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins (PubMed : 19233847). Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock : involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation (PubMed : 24728990). Acts as transcriptional coactivator of progesterone receptor PGR upon progesterone hormone activation (PubMed : 16772533). Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC (By similarity). Required for synaptic remodeling in neurons by mediating ubiquitination and degradation of LAMTOR1, thereby limiting mTORC1 signaling and activity-dependent synaptic remodeling (By similarity). Synergizes with WBP2 in enhancing PGR activity (PubMed : 16772533).. (Microbial infection) Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses.

翻译后修饰

Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates (PubMed : 10373495, PubMed : 16772533, PubMed : 19204938, PubMed : 19233847, PubMed : 19325566, PubMed : 19591933, PubMed : 22645313, PubMed : 24273172, PubMed : 24728990, PubMed : 30020076). Several substrates have been identified including the BMAL1, ARC, LAMTOR1, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B (PubMed : 10373495, PubMed : 19204938, PubMed : 19325566, PubMed : 19591933, PubMed : 22645313, PubMed : 24728990, PubMed : 30020076). Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins (PubMed : 19233847). Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock : involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation (PubMed : 24728990). Acts as transcriptional coactivator of progesterone receptor PGR upon progesterone hormone activation (PubMed : 16772533). Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC (By similarity). Required for synaptic remodeling in neurons by mediating ubiquitination and degradation of LAMTOR1, thereby limiting mTORC1 signaling and activity-dependent synaptic remodeling (By similarity). Synergizes with WBP2 in enhancing PGR activity (PubMed : 16772533).. (Microbial infection) Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses.
See full target information UBE3A
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搭配使用产品

Primary Antibodies

AB272168

Anti-UBE3A antibody [EPR23077-14]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] (AB272168)

  • 0
  • 0
Proteins & Peptides

AB287936

Recombinant Human CD137 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human CD137 protein (Active) (AB287936)

  • 0
  • 0
Proteins & Peptides

AB316630

Recombinant Human Renin Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Renin Protein Standard (His tag) (AB316630)

  • 0
  • 0
Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

  • 0
  • 0

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