重组人UBE2I / UBC9蛋白(Active)
Recombinant human UBE2I / UBC9 protein (Active)
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant human UBE2I / UBC9 protein (Active) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
查看别名
UBC9, UBCE9, UBE2I, SUMO-conjugating enzyme UBC9, RING-type E3 SUMO transferase UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18
- FuncS
Supplier Data
Functional Studies - Recombinant human UBE2I / UBC9 protein (Active) (AB269103)
The specific activity of ab269103 was 200 nmol/min/mg in a UBE2I assay using human SUMO2 and RanGAP1 as substrate.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human UBE2I / UBC9 protein (Active) (AB269103)
SDS-PAGE analysis of ab269103.
反应性数据
序列信息
性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The SUMO conjugating enzyme UBE2I is part of the SUMOylation complex which includes the SUMO E1 enzyme and SUMO E3 ligase. It facilitates the attachment of SUMO proteins to target proteins which can influence protein stability localization and interaction with other proteins. This modification usually regulates important cellular functions such as transcription DNA repair and cell cycle progression.
Pathways
Proteins modified by UBE2I participate in significant biological pathways such as the DNA damage response and cell cycle regulation. In the DNA damage response UBE2I works with proteins such as p53 and BRCA1 to maintain genomic stability. In cell cycle pathways interactions with proteins like cyclin D1 and CDK inhibitors help control cellular proliferation by SUMOylating key regulatory proteins.
特殊说明
形式
Liquid
常规信息
功能
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (PubMed : 26620705).
序列相似性
Belongs to the ubiquitin-conjugating enzyme family.
翻译后修饰
Phosphorylation at Ser-71 significantly enhances SUMOylation activity.
亚细胞定位
Nucleus
靶点信息
搭配使用产品
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com