重组人TWF1/Twinfilin-1蛋白(ab116148)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人TWF1/Twinfilin-1蛋白
参阅全部 TWF1/Twinfilin-1 蛋白酶 -
纯度
> 80 % SDS-PAGE.
purified by using conventional chromatography -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHH SSGLVPRGSH MGSHM MLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD -
预测分子量
32 kDa including tags -
氨基酸
100 to 350 -
标签
His tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab116148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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质谱法
MALDI-TOF -
形式
Liquid -
补充说明
This product was previously labelled as TWF1
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
常规信息
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别名
- A6
- A6 protein tyrosine kinase
- MGC105817
see all -
功能
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. -
组织特异性
Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. -
序列相似性
Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Contains 2 ADF-H domains. -
翻译后修饰
Phosphorylated on serine and threonine residues. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human TWF1/Twinfilin-1 protein (ab116148)15% SDS-PAGE showing ab116148 at approximately 31.5kDa (3µg)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab116148 尚未被引用在任何文献中。