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AB116406

重组人TRF1蛋白

Recombinant Human TRF1 protein

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Recombinant Human TRF1 protein is a Human Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, ELISA, WB, Mass Spec.

查看别名

PIN2, TRBF1, TRF, TRF1, TERF1, Telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1

1 Images
Western blot - Recombinant Human TRF1 protein (AB116406)
  • WB

Unknown

Western blot - Recombinant Human TRF1 protein (AB116406)

10% SDS-PAGE stained with Coomassie Blue (Lane 1), immunobloting with anti-6xHis (Lane 2) serum.

All lanes:

Western blot - Recombinant Human TRF1 protein (ab116406)

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关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

6x His tag N-Terminus GST tag N-Terminus

应用

Mass Spec, ELISA, SDS-PAGE, WB

applications

生物活性

No

访问

P54274

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.4 Constituents: 99% PBS

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"77.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":419,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P54274","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRF1 also known as TERF1 (Telomeric Repeat Binding Factor 1) is an important protein involved in telomere maintenance. This protein has a molecular mass of approximately 55 kDa. TRF1 localizes specifically to telomeres and plays an essential role in shelterin complex formation a specialized protein complex that protects chromosome ends. TRF1 is highly expressed in proliferating cells and tissues especially those with a high rate of cell division.
Biological function summary

TRF1 contributes to the stabilization of telomere structure and regulates telomere length. TRF1 is a part of the shelterin complex which also includes proteins such as TRF2 POT1 TIN2 RAP1 and TPP1. The shelterin complex prevents the telomeres from being recognized as DNA damage and regulates their replicative processes. TRF1 interacts directly with telomeric DNA influencing replication and modulating access to telomerase the enzyme responsible for telomere elongation.

Pathways

TRF1 plays a significant role in telomere maintenance and the cell cycle. The protein is critical in the DNA damage response pathway where it prevents unwanted DNA repair activities at telomeres. In this context TRF1 interacts with proteins like ATM and ATR which are important for signaling and responding to DNA damage. TRF1's interaction with these pathways helps maintain genomic stability and cellular homeostasis.

Alterations in TRF1 function can contribute to cancer and aging-related diseases. High levels or overexpression of TRF1 are frequently observed in certain cancers where it may help tumor cells maintain telomeres and proliferate indefinitely. Conversely lower TRF1 levels are associated with premature aging disorders such as dyskeratosis congenita. In these conditions the connection between TRF1 and TRF2 within the shelterin complex plays an important role in maintaining telomere integrity affecting disease development and progression.
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特殊说明

形式

Liquid

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常规信息

功能

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length (PubMed : 31595153). Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.

翻译后修饰

Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.. ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.. Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length (PubMed : 31595153). Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
See full target information TERF1
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搭配使用产品

Primary Antibodies

AB10579

Anti-TRF2 + TRF1 antibody [TRF-78]

Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (AB10579)

  • 5
  • 10
Primary Antibodies

AB108997

Anti-TRF2 antibody [EPR3517(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (AB108997)

  • 0
  • 0
Primary Antibodies

AB179466

Anti-PML Protein antibody [EPR16792]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PML Protein antibody [EPR16792] (AB179466)

  • 5
  • 5
Proteins & Peptides

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Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

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