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AB134590

重组人TRC40蛋白

Recombinant Human TRC40 protein

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Recombinant Human TRC40 protein is a Human Full Length protein, in the 1 to 348 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

查看别名

ARSA, ASNA1, TRC40, GET3, ATPase GET3, Arsenical pump-driving ATPase, Arsenite-stimulated ATPase, Transmembrane domain recognition complex 40 kDa ATPase subunit, hARSA-I, hASNA-I

1 Images
SDS-PAGE - Recombinant Human TRC40 protein (AB134590)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TRC40 protein (AB134590)

15% SDS-PAGE analysis of 3 µg ab134590

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

O43681

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLIISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ","proteinLength":"Full Length","predictedMolecularWeight":"41.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":348,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43681","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRC40 also known as the transmembrane domain recognition complex subunit is a 41 kDa protein important for targeting and insertion of tail-anchored proteins into the endoplasmic reticulum membrane. It is well-expressed in human tissue especially those with high secretory or membrane protein activity. The protein interacts closely with the cytosolic components and serves as an important part of the guiding mechanism for specific proteins important for cellular function.
Biological function summary

The targeting process involves TRC40 interacting with several tail-anchored proteins. It forms part of a delivery complex required for efficient insertion of these proteins into the correct cellular locations. TRC40 ensures accurate membrane localization firmly supporting cellular physiology and protein homeostasis. This activity is essential for maintaining the protein synthesis balance within the endoplasmic reticulum influencing many cellular metabolic processes.

Pathways

TRC40 operates in the endoplasmic reticulum-associated degradation (ERAD) and secretory pathways. It cooperates with proteins like BAG6 and the Get3 complex acting as routing machinery for tail-anchored protein targeting. These pathways play a critical role in maintaining cellular protein composition by sorting membrane proteins essential for cellular health and stress responses.

TRC40 shows a significant role in neurodegenerative diseases and metabolic syndromes. Misregulation or dysfunction of its targeting process can disrupt protein homeostasis linking it to disorders such as Alzheimer's disease. The ERAD pathway involving TRC40 interacts with proteins like APP and presenilin contributing to the progression of these neurodegenerative conditions. Insights into TRC40's function reveal potential therapeutic targets in tackling related diseases.
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特殊说明

形式

Liquid

附加说明

ab134590 is purified using conventional chromatography techniques.

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常规信息

功能

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling.

序列相似性

Belongs to the arsA ATPase family.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling.
See full target information GET3
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搭配使用产品

Primary Antibodies

AB109493

Anti-IgG4 antibody [EP4420]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (AB109493)

  • 0
  • 0
Primary Antibodies

AB134050

Anti-IgG2 antibody [EPR4418]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (AB134050)

  • 0
  • 0
Primary Antibodies

AB171356

Anti-Human IgG3 antibody [EPR4419] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Human IgG3 antibody [EPR4419] - BSA and Azide free (AB171356)

  • 0
  • 0
Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

  • 0
  • 0

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