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AB114514

重组人Transportin 1/MIP蛋白

Recombinant Human Transportin 1/MIP protein

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Recombinant Human Transportin 1/MIP protein is a Human Fragment protein, in the 25 to 141 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

查看别名

KPNB2, MIP1, TRN, TNPO1, Transportin-1, Importin beta-2, Karyopherin beta-2, M9 region interaction protein, MIP

1 Images
SDS-PAGE - Recombinant Human Transportin 1/MIP protein (AB114514)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Transportin 1/MIP protein (AB114514)

ab114514 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

WB, ELISA, SDS-PAGE

applications

生物活性

No

访问

Q92973

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

This product was previously labelled as Transportin 1.
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序列信息

[{"sequence":"PDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGLILKNNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPKLCSLLDSED","proteinLength":"Fragment","predictedMolecularWeight":"38.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":141,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q92973","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Transportin 1 also known as MIP-1 TRN1 or TNPO1 is a protein involved in nuclear transport mechanisms. It functions as a part of the importin-beta family facilitating the transport of cargo proteins into the nucleus by recognizing nuclear localization signals (NLS). Transportin 1 has a molecular weight of approximately 98 kDa and is expressed widely in various tissues. This protein is essential for the nucleocytoplasmic transport operating predominantly in the cytoplasm where it interacts with its cargoes.
Biological function summary

Transportin 1 is an important component in cellular operation by being part of the nuclear pore complex. It mediates the translocation of proteins across the nuclear envelope. The protein plays a role in not just import but also export of numerous cellular regulators and factors influencing gene expression and cell cycle progression. Being part of such complexes places it at the core of many fundamental cellular processes.

Pathways

The actions of Transportin 1 are integral to nuclear-cytoplasmic transport pathways and the Ran-GTPase pathway. It interacts with the small GTPase Ran which provides the necessary energy for active transport and directionality across the nuclear pore complexes. Additionally Transportin 1 shows functional similarity with importin-alpha which expands its role to include overlap in pathway regulation and cargo specificity further embedding it in complex cellular signaling networks.

Transportin 1 has been linked to neurodegenerative conditions and certain cancers. Its role in improper protein transport potentially contributes to the pathogenesis of neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) where mislocalized proteins accumulate improperly. Transportin 1 also associates with the protein TDP-43 known to play a role in ALS and other similar disorders. In cancer abnormal expression of Transportin 1 can alter nuclear transport dynamics affecting tumor suppressor and oncogene distribution thereby contributing to tumorigenesis and cancer progression.
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特殊说明

形式

Liquid

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常规信息

功能

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed : 24753571). May mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed : 11682607). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones (By similarity). In vitro, mediates nuclear import of SRP19 (PubMed : 11682607). Mediates nuclear import of ADAR/ADAR1 isoform 1 and isoform 5 in a RanGTP-dependent manner (PubMed : 19124606, PubMed : 24753571). Main mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with the karyopherins KPNA1 and KPNA2 (PubMed : 35446349).. (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.

序列相似性

Belongs to the importin beta family. Importin beta-2 subfamily.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed : 24753571). May mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed : 11682607). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones (By similarity). In vitro, mediates nuclear import of SRP19 (PubMed : 11682607). Mediates nuclear import of ADAR/ADAR1 isoform 1 and isoform 5 in a RanGTP-dependent manner (PubMed : 19124606, PubMed : 24753571). Main mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with the karyopherins KPNA1 and KPNA2 (PubMed : 35446349).. (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
See full target information TNPO1
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搭配使用产品

Primary Antibodies

AB10303

Anti-Transportin 1/MIP antibody [D45]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transportin 1/MIP antibody [D45] (AB10303)

  • 4
  • 7
Primary Antibodies

AB181046

Anti-Importin4/Imp4 antibody [EPR13660]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

  • 0
  • 0
Primary Antibodies

AB127165

Anti-Transportin-2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Transportin-2 antibody (AB127165)

  • 0
  • 0
Assay Kits

AB113455

Histone Demethylase (H3K4) Activity Quantification Assay Kit

Functional Studies - Histone Demethylase (H3K4) Activity Quantification Assay Kit (AB113455)

  • 0
  • 0

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