重组人TPX2蛋白(ab119735)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
描述
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产品名称
重组人TPX2蛋白 -
纯度
> 80 % SDS-PAGE.
The purity was determined to be 80% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
110 kDa including tags -
氨基酸
1 to 747 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab119735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.002% PMSF, 0.7% Sodium phosphate, 0.003% DTT, 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride
常规信息
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别名
- C20ORF1
- C20orf2
- Chromosome 20 Open Reading Frame 1
see all -
功能
Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. -
组织特异性
Expressed in lung carcinoma cell lines but not in normal lung tissues. -
序列相似性
Belongs to the TPX2 family. -
发展阶段
Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human TPX2 protein (ab119735)The purity was determined to be >80% by densitometry.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab119735 尚未被引用在任何文献中。