重组人TPPP蛋白(ab167958)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% Densitometry
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: WB, ELISA, MS, SDS-PAGE
描述
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产品名称
重组人TPPP蛋白 -
纯度
> 85 % Densitometry.
ab167958 was purifed using Ni-NTA chromatography and filtered (0.4 µm). -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MKHHHHHHAS MADKAKPAKA ANRTPPKSPG DPSKDRAAKR LSLESEGAGE GAAASPELSA LEEAFRRFAV HGDARATGRE MHGKNWSKLC KDCQVIDGRN VTVTDVDIVF SKIKGKSCRT ITFEQFQEAL EELAKKRFKD KSSEEAVREV HRLIEGKAPI ISGVTKAISS PTVSRLTDTT KFTGSHKERF DPSGKGKGKA GRVDLVDESG YVSGYKHAGT YDQKVQGGK -
预测分子量
25 kDa including tags -
氨基酸
1 to 219 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab167958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
ELISA
Mass Spectrometry
SDS-PAGE
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质谱法
LC-MS/MS -
形式
Lyophilized -
补充说明
Previously labelled as Tubulin Polymerization Promoting Protein.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -80°C.
Constituents: 99% Phosphate Buffer, 0.43% Sodium chloride
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复溶Add 200 µl of deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage.
Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
常规信息
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别名
- 25 kDa brain specific protein
- 25 kDa brain-specific protein
- Brain specific protein p25 alpha
see all -
功能
May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the TPPP family. -
翻译后修饰
Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity. -
细胞定位
Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human TPPP protein (ab167958)12% SDS-PAGE analysis of ab167958:
Lane 1: MWt marker
Lane 2: reduced and boiled sample, 2.5 μg
Lane 3: non-reduced and non-boiled sample, 2.5 μg
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab167958 尚未被引用在任何文献中。
