重组人TNF alpha蛋白(ab52126)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
描述
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产品名称
重组人TNF alpha蛋白
参阅全部 TNF alpha 蛋白酶 -
生物活性
ED50 < 0.05 ng/ml as determined by the cytolysis of murine L929 cells in the presence of actinomycin D.
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纯度
>= 98 % SDS-PAGE.
>98% by SDS-PAGE and HPLC analyses -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
Yes -
性质
Recombinant -
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种属
Human -
序列
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVV PSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSP CQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQV YFGIIAL -
预测分子量
17 kDa -
实际分子量
17 kDa
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技术指标
Our Abpromise guarantee covers the use of ab52126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store under desiccating conditions.
pH: 8.50
Constituents: 0.606% Tris, 0.877% Sodium chloride
Lyophilized in 50 mM Tris, 150 mM NaCl pH 8.5This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute in H2O to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
常规信息
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别名
- APC1
- APC1 protein
- Cachectin
see all -
功能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
疾病相关
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
图片
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Functional Studies - Recombinant human TNF alpha protein (ab52126)
Fig. B. Gel filtration analysis of ab52126: SEC analysis of ab52126 on a Superose 6 Increase 5/150 column at 0.1mL/min in in 50 mM Na-phosphate, 300mM NaCl pH 7.2
Fig. C. ED50 of ab52126: ED50 < 0.05 ng/ml as determined by the cytolysis of murine L929 cells treated with actinomycin using Quick Cell Proliferation Colorimetric Assay Kit Plus
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SDS-PAGE - Recombinant human TNF alpha protein (ab52126)SDS-PAGE (4-20%) of Recombinant human TNF-α Protein loaded under reducing conditions and stained with Coomassie Blue. Lanes are as follows: M - MW markers, 1, 3 and 5 µg purified TNF-α. The protein shows a predicted MW of ~ 17.4 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab52126 尚未被引用在任何文献中。
