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AB125563

重组人TLK1蛋白

Recombinant human TLK1 protein

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Recombinant human TLK1 protein is a Human Full Length protein, in the 1 to 766 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

查看别名

KIAA0137, TLK1, Serine/threonine-protein kinase tousled-like 1, PKU-beta, Tousled-like kinase 1

4 Images
Functional Studies - Recombinant human TLK1 protein (AB125563)
  • FuncS

Unknown

Functional Studies - Recombinant human TLK1 protein (AB125563)

Sample Kinase Assay showing the specific activity of ab125563 to be 6.2 nmol/min/mg.

Functional Studies - Recombinant human TLK1 protein (AB125563)
  • FuncS

Unknown

Functional Studies - Recombinant human TLK1 protein (AB125563)

The specific activity of TLK1 (ab125563) was determined to be 7.1 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human TLK1 protein (AB125563)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TLK1 protein (AB125563)

SDS-PAGE analysis of ab125563.

SDS-PAGE - Recombinant human TLK1 protein (AB125563)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TLK1 protein (AB125563)

SDS PAGE analysis of ab125563

关键信息

纯度

>90% Densitometry

表达系统

Baculovirus infected Sf9 cells

标签

Tag free

应用

SDS-PAGE, WB, FuncS

applications

生物活性

Yes

生物学活性

The specific activity of ab125563 was determined to be 6.2 - 7.1 nmol/min/mg.

访问

Q9UKI8

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY","proteinLength":"Full Length","predictedMolecularWeight":"135 kDa","actualMolecularWeight":null,"aminoAcidEnd":766,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9UKI8","tags":[]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Toutatis-like kinase 1 (TLK1) also known as serine/threonine-protein kinase tousled-like 1 is a kinase that plays a role in DNA repair and chromatin assembly. Its molecular mass is approximately 85 kDa. It is widely expressed in various human tissues with notable expression in the testis and brain. TLK1 primarily functions during DNA replication where it phosphorylates substrates that are vital for maintaining genomic stability.
Biological function summary

TLK1 contributes significantly to chromatin remodeling and DNA repair by influencing histone dynamics. TLK1 often acts in concert with other proteins as part of regulatory complexes modulating DNA accessibility during replication and repair processes. As a kinase it phosphorylates and activates targets like Asf1 a histone chaperone impacting nucleosome assembly.

Pathways

TLK1 plays a significant role in the DNA damage response and cell cycle regulation pathways. It is closely related to the ATM/ATR pathway which is central to the cellular response to DNA damage. TLK1 also interacts with proteins like Asf1 influencing replication-coupled chromatin assembly and cell cycle progression by modifying histones and other structural proteins.

TLK1 has been linked to certain cancers and neurodegenerative disorders. In cancer disruptions in TLK1 activity can lead to genomic instability contributing to tumorigenesis. TLK1 also connects with proteins like ASF1A in these contexts affecting cell proliferation and survival. In neurodegenerative diseases alterations in TLK1 function might impact neurological pathways related to DNA repair though specific mechanisms remain under investigation.
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特殊说明

形式

Liquid

附加说明

Purity determined to be >90% by densitometry.

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常规信息

功能

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.

序列相似性

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.
See full target information TLK1
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