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AB291057

重组人TIMP2蛋白(Active)

Recombinant Human TIMP2 Protein (Active)

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Recombinant Human TIMP2 Protein (Active) is a Human Full Length protein, in the 27 to 220 aa range, with >95%, suitable for FuncS.

查看别名

Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2

3 Images
Functional Studies - Recombinant Human TIMP2 Protein (Active) (AB291057)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human TIMP2 Protein (Active) (AB291057)

Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGLDpa-AR-NH2). ED50 is ≤ 4.9 ng/ml, corresponding to a specific activity of 2.0 x 105 units/mg.

Mass Spectrometry - Recombinant Human TIMP2 Protein (Active) (AB291057)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human TIMP2 Protein (Active) (AB291057)
SDS-PAGE - Recombinant Human TIMP2 Protein (Active) (AB291057)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TIMP2 Protein (Active) (AB291057)

SDS-PAGE analysis of ab291057

关键信息

纯度

>95% SDS-PAGE

标签

His tag C-Terminus

应用

FuncS

applications

生物活性

Yes

生物学活性

Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGLDpa-AR-NH2).

ED50 is ≤ 4.9 ng/ml, corresponding to a specific activity of 2.0 x 105 units/mg.

访问

P16035

不含动物源

No

不含载体蛋白

No

种属

Human

复溶

Reconstitute in PBS

存储溶液

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Proteins & Peptides

AB280945

Recombinant human TIMP1 protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human TIMP1 protein (Active) (AB280945)

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  • 0
Proteins & Peptides

AB285962

Recombinant Human MMP3 protein

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  • 0
Assay Kits

AB270213

Human TIMP2 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TIMP2 ELISA Kit (AB270213)

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Proteins & Peptides

AB196093

Recombinant Mouse Collagen III protein (His tag)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP","proteinLength":"Full Length","predictedMolecularWeight":"21.81 kDa","actualMolecularWeight":"21.81 kDa","aminoAcidEnd":220,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P16035","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

形式
Lyophilized
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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常规信息

功能

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

序列相似性

Belongs to the protease inhibitor I35 (TIMP) family.

翻译后修饰

The activity of TIMP2 is dependent on the presence of disulfide bonds.

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产品实验方案

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靶点信息

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
See full target information TIMP2
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