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AB280945

重组人TIMP1蛋白(Active)

Recombinant human TIMP1 protein (Active)

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Recombinant human TIMP1 protein (Active) is a Human Full Length protein, in the 24 to 207 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

查看别名

CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1

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Mass Spectrometry - Recombinant human TIMP1 protein (Active) (AB280945)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human TIMP1 protein (Active) (AB280945)

Mass determination by ESI-TOF.

Predicted MW is 20708.83 (+/-10 Da by ESI-TOF). Observed MW is 210723.10. Peak at 21033.40 is due to 2 O-galactose.

Functional Studies - Recombinant human TIMP1 protein (Active) (AB280945)
  • FuncS

Supplier Data

Functional Studies - Recombinant human TIMP1 protein (Active) (AB280945)

Functional analysis of ab280945.

Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLG-Dpa-AR-NH2).

ED50 is ≤9.7 ng/ml, corresponding to a specific activity of 1.03 x 105 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3381129-1

HPLC - Recombinant human TIMP1 protein (Active) (AB280945)
  • HPLC

Supplier Data

HPLC - Recombinant human TIMP1 protein (Active) (AB280945)

HPLC analysis of ab280945.

SDS-PAGE - Recombinant human TIMP1 protein (Active) (AB280945)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human TIMP1 protein (Active) (AB280945)

SDS-PAGE analysis of ab280945.

关键信息

纯度

>95% SDS-PAGE

=95%  by HPLC

内毒素水平

<0.005 EU/µg

表达系统

HEK 293 cells

标签

Tag free

应用

HPLC, SDS-PAGE, Mass Spec, FuncS

applications

生物活性

Yes

生物学活性

Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLG-Dpa-AR-NH2).

ED50 is ≤9.7 ng/ml, corresponding to a specific activity of 1.03 x 105 units/mg.

访问

P01033

不含动物源

Yes

不含载体蛋白

No

种属

Human

复溶

Reconstitute in PBS

存储溶液

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB116028

Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (AB116028)

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Proteins & Peptides

AB280347

Recombinant human MMP2 protein (Active)

Premium Bioactive

Functional Studies - Recombinant human MMP2 protein (Active) (AB280347)

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  • 0
Proteins & Peptides

AB285962

Recombinant Human MMP3 protein

  • 0
  • 0
Assay Kits

AB139448

MMP9 Inhibitor Screening Assay Kit (Colorimetric)

Functional Studies - MMP9 Inhibitor Screening Assay Kit (Colorimetric) (AB139448)

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  • 0
Proteins & Peptides

AB291057

Recombinant Human TIMP2 Protein (Active)

Premium Bioactive

Functional Studies - Recombinant Human TIMP2 Protein (Active) (AB291057)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"CTCVPPHPQTAFCNSDLVIRAKFVGTPEVNQTTLYQRYEIKMTKMYKGFQALGDAADIRFVYTPAMESVCGYFHRSHNRSEEFLIAGKLQDGLLHITTCSFVAPWNSLSLAQRRGFTKTYTVGCEECTVFPCLSIPCKLQSGTHCLWTDQLLQGSEKGFQSRHLACLPREPGLCTWQSLRSQIA","proteinLength":"Full Length","predictedMolecularWeight":"20.71 kDa","actualMolecularWeight":"20.72 kDa","aminoAcidEnd":207,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P01033","tags":[]}]
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性能和储存信息

形式
Lyophilized
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
Biological function summary

TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.

Pathways

TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.

TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
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常规信息

功能

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

序列相似性

Belongs to the protease inhibitor I35 (TIMP) family.

翻译后修饰

The activity of TIMP1 is dependent on the presence of disulfide bonds.. N-glycosylated.

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产品实验方案

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靶点信息

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
See full target information TIMP1
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