重组人TIGIT蛋白(Active) (Biotin) (ab271761)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人TIGIT蛋白(Active) (Biotin)
参阅全部 TIGIT 蛋白酶 -
生物活性
Assay conditions: 100 nM of ab271761 was incubated for 1 hour with the indicated concentration of CD155 in a 10 μl reaction in with TIGIT Assay Buffer. Chelate Acceptor beads were added and incubated for 30 mins followed by Strep-donor beads for 30 mins and alpha counts were read.
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纯度
>= 90 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MMTGTIETT GNISAEKGGS IILQCHLSST TAQVTQVNWE QQDQLLAICN ADLGWHISPS FKDRVAPGPG LGLTLQSLTV NDTGEYFCIY HTYPDGTYTG RIFLEVLESS VAEHGARFQI P -
预测分子量
42 kDa -
氨基酸
22 to 141 -
标签
Avi tag C-Terminus , Fc tag C-Terminus -
额外的序列信息
Extracellular domain fused to Fc region of human IgG1.
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偶联物
Biotin
技术指标
Our Abpromise guarantee covers the use of ab271761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
Enzymatically biotin-labeled using Avi-tag™ technology
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- DKFZp667A205
- ENSMUSG00000071552
- FLJ39873
see all -
功能
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. -
组织特异性
Expressed at low levels on peripheral memory and regulatory CD4+ T cells and NK cells and is up-regulated following activation of these cells (at protein level). -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
结构域
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
细胞定位
Cell membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant human TIGIT protein (Active) (Biotin) (ab271761)
SDS-PAGE analysis of 3 µg ab271761.
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Functional Studies - Recombinant human TIGIT protein (Active) (Biotin) (ab271761)Specific activity of ab271761.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271761 尚未被引用在任何文献中。